Breeding method of germplasm containing recessive epistatic non-allelic genes with inhibition effect on blue abortion Ms2 character expression

A technology for suppressing effects and alleles, which is applied in the fields of plant genetic improvement, botanical equipment and methods, and applications, and can solve the problems of inability to achieve cytoplasmic replacement of dominant nuclear sterile lines and short research time.

Pending Publication Date: 2020-11-10
淄博爱民种业有限公司
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AI Technical Summary

Problems solved by technology

[0004] The research time of indigo sterility is relatively short, from Taigu GMMS, dwarf sterility, and indigo sterility, people have been looking for suppressor genes, or epistatic genes, in order to realize the cytoplasmic replacement of dominant genic male sterile li

Method used

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Embodiment Construction

[0024] The present invention will be further explained below in conjunction with specific embodiments.

[0025] 1. The inventors have analyzed and speculated that the relative plants of common wheat may contain inhibitory Ms 2 Non-allelic epigenes (dominant or recessive) of trait expression. If there is a recessive epistasis germplasm, the following conditions will occur when it crosses with the blue-stain germplasm:

[0026] The genetic behavior of blue sterile line conforms to the characteristics of monosomic inheritance. Assuming that a certain white-grained variety has a non-allelic recessive epiele (bb) on the chromosome outside of the monomer addition line for the blue defeat Ms 2 Gene expression has an inhibitory effect, so use this germplasm (bb, ms 2 ms 2 ) Lose to Blue (4D / 4E -BB, Ms 2 ms 2 ) Pollination will occur as follows:

[0027] The genotype of the 4D monosomic female gamete of the blue 4D / 4E translocation addition line is B-Ms 2 , The average transmission rate of...

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Abstract

The invention discloses a breeding method of a germplasm containing recessive epistatic non-allelic genes with an inhibition effect on blue abortion Ms2 character expression, and belongs to the fieldof wheat breeding. According to the breeding method, multiple screened parents containing part of exogenous consanguinity and a local main-cultivated wheat variety are hybridized and subjected to multiple crossing, early-stage hybridization and first-three-generation multiple crossing are adopted, and a mixed seed selection method is adopted for operation according to a multiple-selection and few-obsolescence principle, so that the group scale is enlarged; systematic breeding is conducted from the fourth generation of multiple crossing according to a systematic seed selection procedure; when roguing is carried out, pollinating and backcrossing are carried out on certain test blue abortion plants by using selected plants and selected lines, and the proportions of the plants with fertile blue grains of backcrossed generations of the selected lines are compared to finally breed the Ms2-R102 germplasm conforming to recessive epistatic hereditary characteristic expression. The germplasm material obtained by the breeding method is subjected to blue abortion pollination and then backcrossing, so that half of the blue grains borne on the blue abortion plants are fertile and half of the blue grains are sterile, the current situation that the blue grains borne by the existing blue abortion plants are all sterile is broken through, and the germplasm material is expected to become a rare germplasm for realizing cytoplasm replacement.

Description

Technical field [0001] The invention relates to the field of wheat breeding, in particular to a kind of 2 The breeding method of recessive epistatic non-allelic germplasm with suppressive effect on trait expression. Background technique [0002] Lanpai is a nuclear sterile line marked with blue grains. Its blue grain (4D / 4E), anther abortion (Ms 2 ) Two dominant traits are linked on the 4D chromosome, it is a translocation addition line 4D # monomer. The genetic behavior of blue sterile line conforms to the characteristics of monosomic inheritance. [0003] The sterile gene of the blue sterile line is a single dominant gene Ms from Taigu nuclear sterility 2 The germplasm of this gene has many advantages such as thorough anther abortion and large opening angle of glume. After successive research by the team of Tian Ning and Geng Aimin, Lan Bai has fully demonstrated the many advantages of this approach in the utilization of wheat heterosis, and brought opportunities and hopes for i...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 耿爱民耿一凡于鹏李彩霞耿冬梅张丽刘玉东韩兆普索爱英
Owner 淄博爱民种业有限公司
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