Organ perfusion equipment

Abstract

The invention relates to the technical field of biomedicine, in particular to organ perfusion equipment which is characterized in that a water outlet of a thermostat water bath is communicated with awater inlet of a serpentine condenser pipe through a pipeline, and a water outlet of the serpentine condenser pipe is communicated with a constant-temperature water inlet of the thermostat water baththrough a pipeline; a perfusion bottle is arranged in the thermostat water bath, the perfusion bottle is sequentially communicated with a first peristaltic pump and the serpentine condenser pipe through a pipeline, a water outlet in the upper end of an inner pipe of the serpentine condenser pipe is used for being communicated with an inferior vena cava or a portal vena cava of a liver arranged atthe upper end of the Buchner funnel through a pipeline; and a liquid receiving cup is arranged vertically below the Buchner funnel. The equipment has the beneficial effects that the thermostat water bath is connected with the serpentine condenser pipe to maintain the constant temperature of a perfusate flowing in the pipeline; the peristaltic pump replaces manual operation to propel the perfusate,so that the flow velocity of the perfusate can be stably and accurately controlled, and the perfusion operation can be stably and continuously operated for a long time; a perfused enzyme liquid can be recycled, so that the perfusion operation cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an organ perfusion equipment. Background technique [0002] There are two common cell separation methods, namely mechanical separation and perfusion. The mechanical separation method is rarely used because it will damage the integrity and activity of the cells, and the yield is extremely low; currently, the two-step collagenase perfusion method in vitro or in situ is mainly used to separate cells. Most of the perfusion methods rely on manually pushing the perfusion solution, which is cumbersome, and the perfusion speed is unstable, and the separation and extraction efficiency is low. Although the extracted cells are better than mechanical separation, they still cannot achieve the desired effect. Dealing with large organ livers. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide an organ perfusion equipment that sol...

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a kind of organ perfusion equipment, which solves the problems of continuous manual advancement of the perfusate during the perfusion process, unstable speed and incoherent perfusion, and solves the problems of low perfusion efficiency and low yield

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