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Construction and identification of a systemic plin1 gene knockout animal model

A technology of animal models and construction methods, applied in the biological field, to achieve the effect of improving knockout efficiency

Active Publication Date: 2022-08-02
SHANXI MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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However, there are no reports on the construction and identification of systemic Plin1 gene knockout mouse animal models

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  • Construction and identification of a systemic plin1 gene knockout animal model
  • Construction and identification of a systemic plin1 gene knockout animal model
  • Construction and identification of a systemic plin1 gene knockout animal model

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[0038] The specific embodiments of the present invention will be further described below. In order to facilitate those skilled in the art to understand the present invention, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, as long as various changes are within the spirit and scope of the present invention defined and determined by the claims, These changes are obvious, and all inventions and creations utilizing the inventive concept are included in the protection list.

[0039] Unless otherwise defined, all technical and scientific terms used herein generally have the meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The nomenclature and experimental methods used herein are well known and routinely used in the art. All operations using standard techniques are generally performed in accordance with the instrument consumable manufacturer's produ...

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Abstract

The invention belongs to the field of biotechnology and provides a systemic Plin1 gene knockout animal model construction and identification method thereof. The steps of the construction method are: designing sgRNA sequences according to the sequence before and after exon 2 of the Plin1 gene, constructing a Plin1 gene knockout vector, and performing in vitro transcription to obtain sgRNA; mixing sgRNA and Cas9 protein and then microinjecting it into mouse fertilized eggs to obtain whole body Sexual Plin1 knockout mice. The beneficial effect of this project is that a pair of sgRNA sequences with the highest scores were screened out, which ensured the success rate of the project; a large fragment of exon 2 of the Plin1 gene was knocked out, and the knockout efficiency was improved; the knockout region was designed 3 The two primers can successfully identify various genotypes of mice, avoiding the situation that two primers may not be able to distinguish genotypes when large fragments are knocked out. It provides a convenient, reliable and economical animal model and a good foundation for studying the biological function of PLIN1 and the pathogenesis of obesity-related metabolic diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the construction of a systemic Plin1 gene knockout animal model and an identification method thereof. Background technique [0002] With the rapid economic development and the improvement of people's living standards, obesity has become an increasingly serious global health problem. In recent decades, the global incidence of overweight and obesity has increased significantly, and it is rapidly spreading in both developed and developing countries. It is generally believed that changes in living environment in recent years are directly related to the occurrence of obesity. Unhealthy eating habits, sedentary lifestyles, lack of physical activity, sleep habits, and changes in cultural and demographic backgrounds can all induce obesity. However, in a new foreign study, it was found that genetic factors may play a major role in the occurrence of obesity under the change of li...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/89A01K67/027
CPCC12N15/8509C12N15/89A01K67/0276C07K14/4702C12N2800/107A01K2227/105A01K2267/0362A01K2267/03
Inventor 燕炯许祥冯晨毅刘田福陈朝阳高学坤
Owner SHANXI MEDICAL UNIV
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