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Sudden cardiac death susceptibility detection kit based on STIM1 gene insertion and deletion polymorphic sites

A detection kit and sudden cardiac death technology, applied in the field of detection, can solve problems such as predicting SCD susceptibility

Inactive Publication Date: 2020-12-11
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the prior art, there is no research report on the correlation between the rs3061890 indel polymorphism and SCD, and there is no related report on predicting the susceptibility to SCD by detecting the indel polymorphism site in the 3'UTR of the STIM1 gene

Method used

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  • Sudden cardiac death susceptibility detection kit based on STIM1 gene insertion and deletion polymorphic sites
  • Sudden cardiac death susceptibility detection kit based on STIM1 gene insertion and deletion polymorphic sites

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Embodiment 1

[0023] Embodiment 1: the use of detection kit

[0024] Step 1: Extraction of DNA template

[0025] Genomic DNA from peripheral blood was extracted using a blood genomic DNA extraction system (non-centrifugal column type).

[0026] Step 2: PCR reaction - replication of the target fragment

[0027] Use a PCR assay kit for SCD susceptibility, which contains the following primers:

[0028] SEQ ID No.1: 5'-TTCTGCTGCTCGCCGCTCTT-3', the Tm value is 64°C;

[0029] SEQ ID No.2: 5'-GCTGTCAGCTCCGCAGATTACTACAC-3', Tm value is 64°C;

[0030] The primer pair can specifically amplify the fragment containing the polymorphism of the insertion-deletion site of rs3061890 in the STIM1 gene.

[0031] The total volume of the PCR reaction system is 10ul, including: 1μl DNA template, 0.04μl each of two 50μM specific primer pairs, 0.08μl 2.5U / μl Taq DNA polymerase; 0.2μl 2.5mM dNTP mixture; 25mM MgCl 2 Solution 0.6 μl; 10×PCR reaction buffer 1 μl; make up with deionized water; react on the Eppend...

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Abstract

The invention discloses a sudden cardiac death susceptibility detection kit based on STIM1 gene insertion and deletion polymorphic sites. A specific primer pair contained in the kit is designed for aninsertion and deletion site No. rs3061890 on an STIM1 gene, DNA fragments containing the site can be specifically amplified, and different genotypes are recognized by detecting the mobility of the fragments with different lengths in capillary electrophoresis. It is found that in combination with our case control study, it can be considered that a person whose insertion and deletion site No. rs3061890 on the STIM1 gene of a detected DNA carries a deletion type allele is of an SCD susceptible type. Therefore, the technology can play a role in predicting the SCD susceptibility of an individual by detecting the genotype of the insertion and deletion site No. rs3061890 on the STIM1 gene of the individual; the subject group proves for the first time that a tetrabasic group (TCTC) insertion anddeletion polymorphism (rs3061890) in 3'UTR of the STIM1 gene is significantly associated with the risk of suffering from SCD; and the frequency distribution of the polymorphism in the Asian populationis that an insertion type is 0.669, and a deletion type is 0.331.

Description

technical field [0001] The invention relates to a sudden cardiac death susceptibility detection kit based on an insertion-deletion polymorphism site of STIM1 gene, which belongs to the technical field of detection. Background technique [0002] Sudden cardiac death (sudden cardiac death, SCD) refers to sudden unexpected death caused by cardiac causes, manifested as sudden loss of consciousness and sudden circulatory and respiratory arrest in a short period of time. "Short-term" is defined as less than 1 hour in the case of witnesses, and 24 hours in the absence of witnesses. Epidemiology shows that 40.7 people per 100,000 people die from SCD every year in China. Considering the huge population base in my country, the number of SCD cases is very large. A large number of previous studies have shown that the cause of death in SCD is mostly coronary atherosclerotic heart disease or fatal arrhythmia. The former is more common in elderly people, while the latter is more common i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125C12Q2563/107
Inventor 何艳张晴于欢高玉振
Owner SUZHOU UNIV
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