In-vitro constructed method for accurately predicting medication of patients with cancers

A patient and cancer technology, applied in the field of biomedicine, can solve the problem of not being able to know that the drug is effective in patients, and achieve the effect of shortening the digestion time, improving the success rate of culture, and maintaining the same degree of digestion

Inactive Publication Date: 2021-01-05
普罗布诺(重庆)生物技术有限公司
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Problems solved by technology

[0008] The time period of clinical medication is usually in years, and it is imposs...

Method used

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Embodiment Construction

[0048] A method for accurately predicting medication for cancer patients constructed in vitro, the method comprising the following steps:

[0049] 1) Construction of intestinal cancer organoids

[0050] Using cancer tissues obtained from patients with colon cancer, rectal cancer or colorectal cancer through surgery or biopsy to cultivate purer intestinal cancer organoids; this step specifically includes the following steps:

[0051] 1.1) Cleaning: Take out the tissue and put it into an ordinary petri dish and wash it with PBS for 3-4 times until there is no bloody turbidity and the liquid is basically colorless;

[0052] 1.2) Grinding: After washing, transfer the tissue to a clean low-adsorption dish and chop it with a sterile blade to make it evenly loose and non-adhesive;

[0053] 1.3) Digestion: Take 4ml of tissue digestion solution to digest the chopped tissue, pipette and mix well, and digest in a constant temperature water bath at 37°C for 30 minutes;

[0054] 1.4) Scr...

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Abstract

The present invention discloses an in-vitro constructed method for accurately predicting medication of patients with cancers. The method comprises the following steps of: 1) constructing an intestinalcancer organoid, namely culturing a purer intestinal cancer organoid by using cancer tissues obtained by operations or biopsies of patients with colon cancer, patients with rectal cancers or patientswith colorectal cancers; and 2) carrying out a large-flux drug screening test and carrying out a high-flux drug sensitivity detection by taking a micro organ as a unit. The in-vitro constructed method for accurately predicting the medication of the patients with the cancers has advantages that grinding the tissues enables digestion time to be shortened and a digestion degree is also consistent; mild digestive juice reduces damages of the step to cells; different pore diameters are used for filtering to screen the required cells more accurately; immune cells are removed to obtain purer cancercells; a self-made culture medium better conforms to components required by growth of intestinal cancer organs and a culture success rate is increased; and medicine screening is carried out by takingthe organoid as a unit, so that responses of the cancer tissues in vivo after clinical medication are better simulated.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to an in vitro constructed method for accurately predicting medication for cancer patients. Background technique [0002] At present, there are mainly the following methods for predicting drug use in cancer patients: [0003] 1) Genetic diagnosis [0004] Clinically, there are many cases where the targeted drugs used after genetic diagnosis are ineffective. It may be due to the complex environment in the body, gene mutation is not the main pathogenic factor or other unknown reasons, so the predictability of genetic diagnosis for patients' medication is not high. [0005] 2) Cancer cell line drug experiment [0006] The in vitro growth environment of cell lines is significantly different from the in vivo environment, and most of them are commercial general-purpose cell lines with a low degree of personalization. They cannot accurately simulate the actual tumor response to drugs, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5011G01N33/5088G01N2500/00
Inventor 陈益莹
Owner 普罗布诺(重庆)生物技术有限公司
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