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X-STR fluorescence amplification system, kit and application

A technology of X-STR and amplification system, which is applied in the field of X-STR fluorescence amplification system to achieve the effect of improving accuracy

Active Publication Date: 2021-01-08
SUZHOU MICROREAD GENETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology mainly detects a small number of STR sites on the X chromosome, and there are certain deficiencies in genetic polymorphism, individual recognition and detection accuracy. Therefore, it is necessary to develop an X-STR kit with high performance and strong individual recognition ability

Method used

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  • X-STR fluorescence amplification system, kit and application
  • X-STR fluorescence amplification system, kit and application
  • X-STR fluorescence amplification system, kit and application

Examples

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Embodiment Construction

[0026] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0027] 1) Screening of X-STR loci:

[0028] The statistics of the genetic data of the 28 X-STRs screened by the present invention are shown in the results (Table 1).

[0029] Table 1: Statistical table of genetic data of 28 X-STRs used in the present invention

[0030]

[0031]

[0032] 2) Primer design:

[0033] The primers are designed using software such as Prime Premier5 and NCBI Blast. When designing primers, it should be ensured that the Tm value of each primer is within the range of (60±2)°C, and the amplification efficiency is close to that of the amplification product of each pair of primers. different eno...

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Abstract

The invention discloses an X-STR fluorescence amplification system, a kit and application. The amplification system comprises 29 pairs of primers corresponding to 28 X-STR loci on X chromosome and anAmel locus, and the nucleotide sequences of the amplification system are shown as SEQ ID NO. 1-58. The corresponding 28 STR loci on X chromosome designed in the amplification system comprise a plurality of linkage groups on X chromosome, the number of detection loci is large, and the detection loci are distributed on the short arm, the centromere and the long arm of the X chromosome, so that the detection accuracy can be effectively improved. In particular, the amplification system can better meet the high requirements of public security and judicial organs for X-STR testing.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to an X-STR fluorescence amplification system, a kit and an application. Background technique [0002] Short tandem repeats (Short tandem repeats, STR) are currently the mainstream genetic markers used in forensic DNA typing research. Human genome STR (short tandem repeat sequence) is a DNA genetic marker formed by tandem repeats with a few bases as the core unit. The distinction between different races, different populations and even different individuals is through the difference in core unit sequence and repeat number, which also constitutes the genetic polymorphism of STR. In the genome, there is an STR site every 15-20kb on average, accounting for 10% of the genome, mostly in non-coding regions and introns, the repeat unit is 2-6bp, the number of repeats is 10-60 times, and the fragment size In 70 ~ 500bp, and was co-dominant inheritance according to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6876C12Q1/6858C12N15/11
CPCC12Q1/6876C12Q1/6858C12Q2600/156C12Q2531/113C12Q2563/107C12Q2527/125
Inventor 于在亮杨凡张德露冯晓琴陈初光
Owner SUZHOU MICROREAD GENETICS
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