Follicle-stimulating hormone receptor activity detection kit and detection method thereof

Abstract

The invention discloses a follicle-stimulating hormone receptor activity detection kit and a detection method thereof. The detection kit comprises two pairs of amplification primers and a multiplex PCR mixed reaction solution, wherein the amplification primers are used for amplifying obese gene polymorphic sites and comprise rs6165 sites and rs6166 sites, and each pair of primers respectively corresponds to upstream and downstream regions of one SNP site. According to the follicle-stimulating hormone receptor activity detection kit and the detection method thereof, a multiplex PCR amplification method is adopted, two important SNP sites having high relevancy with FSHR activity are amplified at the same time, the amplified site combination can be flexibly selected according to actual needsto achieve the function of one kit with multiple functions, and primers in the kit are high in specificity and low in mismatch rate. The primers are reasonable in length and Tm value design, and the amplified product is convenient to detect and isolate. Multiple target products are amplified once, so that the amplification efficiency can be greatly improved, and the amplification cost can be reduced. The detection kit can be extended to other SNP site detection, and has a good popularization value.

Description

technical field [0001] The invention relates to a detection kit for follicle-stimulating hormone receptor activity and a detection method thereof, belonging to the field of detection kits. Background technique [0002] After women enter puberty, the ovary develops eggs and ovulates under the action of follicle stimulating hormone (FSH) from the pituitary gland. After the combination of follicle stimulating hormone receptor (FSHR) and FSH, it stimulates the proliferation and differentiation of granulosa cells, and secretes follicular fluid to make follicles growth and development. FSHR inactivating mutations mainly cause primary and secondary amenorrhea and infertility with high gonadotropin levels in women, and may be accompanied by ovarian dysplasia. FSHR activating mutations mainly cause women to be prone to ovarian overstimulation during natural pregnancy. Syndrome (OHSS). At present, the mutations about FSHR recorded in HGMD (the Human Gene Mutation Database) are all m...

AI Technical Summary

Problems solved by technology

At present, the mutations about FSHR recorded in HGMD (the Human Gene Mutation Database) are all missense point mutations, and there are only 13 kinds. However, according to the detection of samples from clinical patients, it was found that new mutations did occur

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