Preparation method of mouse orthotopic transplantation liver cancer model

Abstract

The invention relates to a preparation method of a mouse orthotopic transplantation liver cancer model, in particular to a preparation method of a mouse orthotopic liver cancer model suitable for liver cancer occurrence and development mechanisms and antitumor related research. After a mouse is anesthetized, a median incision in the upper abdomen enters the abdomen, a pair of atraumatic hemostaticforceps performs first hepatic portal occlusion, 0.05 ml of liver cancer cells are injected into the left liver lobe with a No. 301/2 curved syringe needle, an electrocoagulation pen solidifies a needle eye, the hepatic portal occlusion forceps are loosened after no active bleeding occurs, and the abdomen closure is carried out. The method has the technical key points that the hepatic portal occlusion is carried out before orthotopic injection, the hepatic blood flow is controlled, so that the injected tumor cells locally reside in the liver tissue, and the defects that the tumor cells flow out due to the blood flow, the implantation is not limited, the tumor formation rate is low and the like are overcome; and hemorrhagic shock death and intraperitoneal implantation of tumors caused by excessive bleeding of the needle eye are remarkably reduced, and the success rate of modeling is remarkably increased. The model is very valuable for researching a liver cancer occurrence and development mechanism and evaluating the experimental drug treatment effect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for preparing a mouse orthotopic transplanted liver cancer model suitable for studies on the occurrence and development mechanism of liver cancer and anti-tumor related research. Background technique [0002] my country is the hardest-hit area of ​​liver cancer, and the number of new cases of liver cancer each year accounts for more than 55% of the total number of cases in the world, and there is an increasing trend year by year. The onset of liver cancer is insidious, and there are usually no obvious symptoms in the early stage. At the time of clinical diagnosis, it is mostly in the middle and late stages, and the chance of radical surgery is lost. Despite the continuous improvement of medical technology and surgical techniques in recent years, the radical resection rate of liver cancer is still only about 30%, and recurrence and metastasis are easy afte...

AI Technical Summary

Problems solved by technology

However, the continuous bleeding at the inoculation site after the inoculation is completed (serious cases will lead to hemorrhagic shock death of experimental animals) and the leakage of cancer tissue (or cells) into the abdominal cavity causing peritoneal dissemination are two major problems to be solved by this technology.

[0007] In summary, the three existing orthotopic liver cancer animal models all have certain deficiencies, and cannot fully meet the current needs of basic and clinical translational research on liver cancer.

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