Application of dendritic cell with ATPIF1 gene knock-down expression

A dendritic cell and gene technology, applied in the field of biomedicine, can solve the problems of ineffective tumor antigen presentation to lymphocytes, weak tumor cell antigenicity, and low function of antigen presenting cells.

Pending Publication Date: 2021-01-29
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Patients with malignant tumors have low immunity and lack a strong anti-tumor immune response. The reason is that the antigenicity of tumor cells is weak, and the function of antigen-presenting cells is low, so that tumor antigens cannot be effectively presented to lymphocytes.

Method used

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  • Application of dendritic cell with ATPIF1 gene knock-down expression
  • Application of dendritic cell with ATPIF1 gene knock-down expression
  • Application of dendritic cell with ATPIF1 gene knock-down expression

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Experimental animals: All mice (8-12 weeks old) were bred and provided by our laboratory, ATPIF1 - / - Mice were obtained by knocking out the ATPIF1 gene by CRISPR / Cas9 technology, and the WT mice in the control group were ATPIF1 + / - ATPIF1 from mated littermates + / + normal mice. Mice were raised in an SPF-grade environment, with 12 hours of light and dark alternation, ambient temperature 22-26°C, humidity 40%-60%, fed with low-fat diet (fat = ≥ 4% kcal), and free access to food and water.

[0020] Extraction, induction and culture of mouse bone marrow dendritic cells (BMDCs)

[0021] 1. Obtaining mouse bone marrow cells

[0022] 1) Two wild-type mice and two ATPIF1- / - mice (8-12 weeks old) were killed by cervical dislocation, and all femurs and tibias were surgically removed, and the bones around the bones were removed with scissors and forceps. Remove the muscle tissue as much as possible, and put it into pre-cooled sterile PBS; Note: Do not damage the bone;

[002...

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Abstract

The invention relates to the fields of biotechnology and medicine, and provides a dendritic cell (DC) with ATPIF1 gene knock-down expression. The ATPIF1 gene is knocked down in the dendritic cell, sothat the antigen uptake, processing and presentation capabilities of the dendritic cell in the immune process are enhanced, the initial T cell is effectively activated, the effect function of the T cell is improved, and the killing effect of the T cell on tumor cells is enhanced. The dendritic cell with ATPIF1 knock-down expression has a potential application value in the aspect of preparing immunotherapy drugs for tumors.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a method for enhancing the antigen processing and presenting ability of dendritic cells and the application of the dendritic cells obtained by the method in tumor immunotherapy drugs. [0002] technical background [0003] Dendritic cells (DC) are professional antigen-presenting cells (Antigen presenting cells, APCs) with the strongest function in the body, which can efficiently ingest, process and present antigens, and immature DCs have a strong ability to migrate Mature DC can effectively activate naive T cells, and is at the center of initiating, regulating, and maintaining immune responses. Dendritic cells are the strongest antigen-presenting cells in the body known so far, and can be divided into mature DC (mature DC, mDC) and immature DC (immature DC, imDC) in terms of differentiation degree and maturity state. Immature DCs exist in the non-lymphoid tissues o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/15A61P35/00C12N5/10
CPCA61K35/15A61P35/00C07K14/4703C12N5/0639C12N2510/00
Inventor 钟根深张姣姣叶建平王辉
Owner XINXIANG MEDICAL UNIV
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