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System and method for cell imaging, analysis and measurement

A technology of imaging and imaging holes, applied in the field of measurement and analysis of biological samples, can solve the problem of not being able to provide time saving

Pending Publication Date: 2021-02-26
NEXCELOM BIOSCIENCE LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it still takes 2 to 3 minutes per sample, so may not provide sufficient time-saving benefit

Method used

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  • System and method for cell imaging, analysis and measurement
  • System and method for cell imaging, analysis and measurement
  • System and method for cell imaging, analysis and measurement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0121] Exemplary systems have been constructed and used to validate the enumeration of CHO cells stained with trypan blue, PBMCs stained with AOPI, and Jurkat cells stained with AOPI. It was used to validate the cell count, cell size and viability measurements and capabilities of this exemplary system.

[0122] CHO cell counts with manual pipetting

[0123] In CD CHO medium (Gibco) supplemented with 1X Glutamax and 1X HT supplement, at 37 °C and 5% CO 2 CHO cells were cultured under. CHO cells were collected from the flask and poured into disposable tanks. Pipette 50 μL of CHO cells into the sample introduction port of the cytometry plate using a multichannel pipette (12-channel). Repeat the process to fill all 24 counting chambers. The exemplary system was then used to directly count the number of CHO cells using brightfield imaging at each counting chamber, which was then automatically converted and output cell concentration as a result along with output cell size ( ...

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PUM

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Abstract

The invention provides novel sample chambers, sample analysis units, multi-well plates, and imaging and analytical systems as well as methods for accurate, efficient and high-throughput imaging, measurement and analysis of diverse types of biological cells to obtain information such as cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

Description

[0001] Priority claims and related patent applications [0002] This application claims U.S. Provisional Application Serial No. 62 / 633,520 filed February 21, 2018, U.S. Provisional Application Serial No. 62 / 673,204 filed May 18, 2018, and U.S. Provisional Application Serial No. 62 / 673,204 filed February 1, 2019 62 / 800,353, the entire contents of each of these provisional applications are hereby incorporated by reference in their entirety. technical field [0003] The present invention generally relates to the measurement and analysis of biological samples. More particularly, the present invention relates to novel sample chambers, sample analysis units, multi-well plates, systems and methods thereof for precise, efficient and high-throughput imaging, measurement and analysis of various types of biological cells, e.g., cell counting, cell Size, cell concentration, cell subpopulations, cell morphology and cell viability measurements. Background technique [0004] An importan...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M3/00G01N1/10G01N33/483G01N35/00
CPCB01L3/5085B01L2300/087B01L2300/0829B01L2300/0654B01L3/502715G01N2015/1486G01N15/1468G01N2015/1006G01N21/6452G01N33/487B01L2200/10G01N15/147G01N33/4875
Inventor P·Y·李J·丘L·L·常J·R·哈斯库普T·F·史密斯T·R·索博列维斯基N·N·肖
Owner NEXCELOM BIOSCIENCE LLC