Aryl sulfatase and glucoside aldehydic acid enzyme inhibitor, preparation method therefor and application of aryl sulfatase and glucoside aldehydic acid enzyme inhibitor
An aryl sulfatase and glucuronidase technology, applied in the medical field, can solve the problems of expensive inhibitors and high 50% inhibitory concentration
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Embodiment 1
[0020] Take two 3mL Tris-HCl buffer solution (pH=5.8) in two glass test tubes, denoted as test tube 1 and test tube 2. Proceed as follows for test tube 1 and test tube 2:
[0021] Test tube 1:
[0022] Add 4.02U of arylsulfatase to test tube 1, then add sodium hypochlorite (0.28mol / L) so that the concentration of hypochlorite in the Tris-HCl buffer solution is 20μmol / L, and then add paranitrate according to the standard of 800μmol / L phenol-sulfuric acid conjugates, the glass test tube was placed in a 37 ° C water bath for 1 hour, and then the reaction was terminated by adding 2 mL of 0.5 mol / L sodium hydroxide, using standard methods (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34:225-229) assay for arylsulfatase activity.
[0023] Test tube 2:
[0024] Add 3.29U of glucuronidase to test tube 2, then add sodium hypochlorite (0.28mol / L), so that the concentration of hypochlorite in the Tris-HCl buffer solution is 20 μmol / L, and then add par...
Embodiment 2
[0026] Take two 3mL Tris-HCl buffer solution (pH=5.8) in two glass test tubes, denoted as test tube 1 and test tube 2. Proceed as follows for test tube 1 and test tube 2:
[0027] Test tube 1:
[0028] Add 4.02U of arylsulfatase to test tube 1, then add sodium hypochlorite (0.28mol / L) so that the concentration of hypochlorite in the Tris-HCl buffer solution is 100μmol / L, and then add paranitrate according to the standard of 800μmol / L phenol-sulfuric acid conjugates, the glass test tube was placed in a 37 ° C water bath for 1 hour, and then the reaction was terminated by adding 2 mL of 0.5 mol / L sodium hydroxide, using standard methods (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34:225-229) assay for arylsulfatase activity.
[0029] Test tube 2:
[0030] Add 3.29U of glucuronidase to test tube 2, then add sodium hypochlorite (0.28mol / L), so that the concentration of hypochlorite in the Tris-HCl buffer solution is 100 μmol / L, and then add p...
Embodiment 3
[0032] Take two 3mL Tris-HCl buffer solution (pH=5.8) in two glass test tubes, denoted as test tube 1 and test tube 2. Proceed as follows for test tube 1 and test tube 2:
[0033] Test tube 1:
[0034] Add 4.02U of arylsulfatase to test tube 1, then add sodium hypochlorite (0.28mol / L), so that the concentration of hypochlorite in the Tris-HCl buffer solution is 200μmol / L, and then add p-Nitrate according to the standard of 800μmol / L phenol-sulfuric acid conjugates, the glass test tube was placed in a 37 ° C water bath for 1 hour, and then the reaction was terminated by adding 2 mL of 0.5 mol / L sodium hydroxide, using standard methods (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34:225-229) assay for arylsulfatase activity.
[0035] Test tube 2:
[0036] Add 3.29U of glucuronidase in test tube 2, then add sodium hypochlorite (0.28mol / L), so that the concentration of hypochlorite in the Tris-HCl buffer solution is 200 μmol / L, and then add pa...
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