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A long-chain non-coding gene and its related biological materials and applications

A long-chain non-coding, biomaterial technology, applied in the biological field, can solve the problem that the potential role of immune regulation has not been elucidated

Active Publication Date: 2022-05-10
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the preparation and storage of platelet concentrates, a large number of P-EVs will be generated. P-EVs, as an important medium for intercellular information transmission, carry a large number of genetic information in platelets, among which long non-coding RNA (LncRNA) in the cell It plays an important regulatory role in physiological processes such as differentiation, proliferation, and apoptosis. The potential role of infusion of P-EVs in vitro on the regulation of the body's immunity has not yet been elucidated

Method used

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  • A long-chain non-coding gene and its related biological materials and applications
  • A long-chain non-coding gene and its related biological materials and applications
  • A long-chain non-coding gene and its related biological materials and applications

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Experimental program
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Effect test

Embodiment 1

[0072] 1. Extraction and purification of P-EV

[0073] Enze Kangtai’s Exosupur exosome purification kit was used.

[0074] The machine-collected platelets obtained on the same day were centrifuged at 1500g at 4°C for 20 minutes to remove cells in the blood, and the supernatant was collected and centrifuged at 3000g at 4°C for 15 minutes to collect the supernatant to obtain platelet-free plasma. Filter the platelet-free plasma with a 0.22um microporous membrane, and the specific steps are as follows:

[0075] Column equilibration: Before the experiment, place the exclusion column at room temperature for more than 30 minutes to fully equilibrate the column temperature to room temperature. Place the waste liquid collection tube under the exclusion column, remove the top and bottom covers of the column, recover the column sealing solution on the column, and add no less than 20mL of PBS from the top to rinse the column.

[0076] Remove the bottom cover of the column, use a pipett...

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Abstract

The invention discloses a long-chain non-coding gene, which is a nucleic acid molecule after knocking out position 1090-1149 of the MIR4435-2HG gene or a nucleic acid molecule after knocking out position 1235-1294 of the MIR4435-2HG gene. The experiment of introducing the long-chain non-coding gene of the present invention into macrophages proves that compared with the macrophage expressing the MIR4435-2HG gene, the macrophage expressing the long-chain non-coding gene of the present invention significantly delays Apoptosis is expected to be applied in blood transfusion and preparation of drugs for treating cancer.

Description

technical field [0001] The invention relates to a long-chain non-coding gene and related biological materials and applications in the field of biotechnology. Background technique [0002] Platelet concentrate (PC) plays an important role in clinical blood transfusion therapy. Platelet transfusion is required in the treatment of patients with thrombocytopenia caused by massive blood loss and chemotherapy, radiotherapy and other treatments. Because platelets are difficult to preserve in vitro and carry out functional experiments, the discovery of human platelet-derived extracellular vesicles (P-EVs) provides a solution for the study of platelet function. During the preparation and storage of platelet concentrates, a large number of P-EVs will be generated. P-EVs, as an important medium for intercellular information transmission, carry a large number of genetic information in platelets, among which long non-coding RNA (LncRNA) in the cell Differentiation, proliferation, apopto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N5/10A61K31/7088A61P7/06A61P35/00A61P39/00
CPCC12N15/113C12N5/0645A61K31/7088A61P7/06A61P35/00A61P39/00C12N2310/10C12N2510/00
Inventor 张艳宇邓江张骞张阳阳吕丽萍马平
Owner ACADEMY OF MILITARY MEDICAL SCI