A calibration device for optical traceability standard device of fluorescent quantitative PCR instrument

A technology for fluorescence quantitative and calibration devices, applied in biochemical cleaning devices, enzymology/microbiology devices, specific-purpose bioreactors/fermenters, etc., can solve the problems of lack of methods and corresponding equipment, and achieve rigor Be scientific, ensure accuracy, and ensure effectiveness

Active Publication Date: 2021-10-29
北京林电伟业电子技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no method and corresponding equipment for the superior traceability of the optical traceability standard device of the fluorescent quantitative PCR instrument using the optical physical method.

Method used

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  • A calibration device for optical traceability standard device of fluorescent quantitative PCR instrument
  • A calibration device for optical traceability standard device of fluorescent quantitative PCR instrument
  • A calibration device for optical traceability standard device of fluorescent quantitative PCR instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1. A calibration device for an optical traceability standard device for a fluorescent quantitative PCR instrument

[0046] as attached figure 1 As shown, the calibration device in this embodiment adopts a cabinet design, and a set of universal wheels 9 are arranged at the bottom of the cabinet to facilitate the movement of the device. The cabinet is divided into measurement closed environment area 1 and automation control device area 2. A human-computer interaction module is arranged outside the cabinet, and the human-computer interaction module includes a control panel 7 and a display module 8 .

[0047] Measuring the closed environment area 1 adopts a light-proof design to form a closed and dark environment. A sealed warehouse (closed cabinet) can be used, and other dark environment conditions such as darkrooms or cassettes can also be used. A three-axis positioning system 3 , an optical measurement unit 4 and a temperature condition simulation device 5 a...

Embodiment 2

[0058] Embodiment 2. A calibration device for an optical traceability standard device for a fluorescent quantitative PCR instrument

[0059] In this embodiment, the calibration device is divided into a measurement closed environment area 1 and an automatic control device area 2 . A human-computer interaction module is arranged outside the cabinet, and the human-computer interaction module includes a control panel 7 and a display module 8 .

[0060] Measuring the closed environment area 1 adopts a light-proof design to form a closed and dark environment. A sealed warehouse (closed cabinet) can be used, and other dark environment conditions such as darkrooms or cassettes can also be used. A three-axis positioning system 3 and an optical measurement unit 4 are arranged in the measurement enclosed environment area 1 .

[0061] The optical measurement unit 4 includes an optical sensor and a CCD field of view monitoring device; the optical sensor is used to measure the optical para...

Embodiment 3

[0069] Embodiment 3. A calibration device for optical traceability standard device of fluorescent quantitative PCR instrument

[0070]The calibration device in this embodiment adds a standard light source on the basis of the calibration device in Embodiment 1, and the standard light source is set in the measurement closed environment area 1 and within the detection range of the optical measurement unit 4 . By setting a standard light source, the numerical value of the device under test can be compared, and then the measurement error can be corrected. By adding a standard light source, this setting can realize the self-calibration of the calibration device of the optical traceability standard device of the fluorescent quantitative PCR instrument, improve the measurement accuracy of the optical sensor and ensure that the measured device is more accurate.

[0071] In addition, in this embodiment, the imaging optical sensor is used for photographic measurement, and all points in t...

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Abstract

This application discloses a calibration device for the optical traceability standard device of a fluorescent quantitative PCR instrument, which includes a measurement closed environment area and an automatic control device area; Shaft positioning system, optical measurement unit; automatic control device is set in the automatic control device area. The device aligns the optical sensor with the position and height of the light source of the optical traceability standard device of the fluorescent quantitative PCR instrument by means of an automatic displacement and positioning control device under the conditions of a closed and dark environment and a simulated temperature environment, and the light source changes in the process. The optical parameters are measured, and the measurement spectrum range is 380-780nm, the resolution is 1.56nm / pixel, the spectral accuracy is ±1nm, and the brightness accuracy is within ±2%. And through the CCD field of view monitoring device, the collected field of view is photoelectrically converted and presented to the display module for positioning and visualization operations.

Description

technical field [0001] The present application relates to the field of calibration of optical devices, in particular to a calibration device for an optical traceability standard device of a fluorescent quantitative PCR instrument. Background technique [0002] Fluorescence quantitative PCR instrument is widely used in medical testing, clinical diagnosis, research and development and manufacturing of in vitro diagnostic reagents, molecular biology, biomedicine, entry-exit inspection and quarantine and scientific research. Its core technology refers to the addition of fluorescent bases to the PCR reaction system. The group uses the accumulation of fluorescent signals to monitor the entire PCR reaction process in real time, and finally uses the standard curve to quantitatively analyze the unknown template. This method mainly involves two parts: thermal and optical, and its optical parameters and instrument optical performance are currently mainly Calibration and traceability ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/38C12M1/36C12M1/00C12Q1/6851
Inventor 李征付志勇张辰孙俊峰周文张峻万全寿赵丹丹
Owner 北京林电伟业电子技术有限公司
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