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Method for culturing phyllostachys edulis protoplast and inducing cell wall regeneration and application of method

A protoplast, induced cell technology, applied in the field of plant tissue culture, can solve the problems of low efficiency, poor repeatability, difficulty in regenerating cell walls, etc., and achieve the effect of high success rate, high repeatability, quality and yield improvement

Active Publication Date: 2021-11-05
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the methods for culturing moso bamboo protoplasts mentioned in existing research reports still have many characteristics such as poor repeatability, low efficiency, and difficulty in regenerating cell walls.
[0014] In addition, the current research on the cell wall of Moso bamboo is mostly focused on analyzing the microstructure and chemical composition of the cell wall, the formation of the secondary cell wall, and the function of a single gene family, etc., lacking the overall monitoring and systematic research on the protoplast culture and cell wall regeneration process

Method used

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  • Method for culturing phyllostachys edulis protoplast and inducing cell wall regeneration and application of method
  • Method for culturing phyllostachys edulis protoplast and inducing cell wall regeneration and application of method
  • Method for culturing phyllostachys edulis protoplast and inducing cell wall regeneration and application of method

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Embodiment 1

[0071] The present embodiment provides a method for cultivating moso bamboo protoplasts and inducing cell wall regeneration, the specific steps are as follows:

[0072] (1) Select the solid moso bamboo seeds of the current year, remove the seed coat, select the seeds with plump grains, smooth surface and no mildew, brew in running water for 24 hours, soak in 200mg / L gibberellin solution for another 2 hours, and cut off the tail. In the ultra-clean operation bench, soak the seeds with 75% alcohol for 1 min, and then wash them with sterile water for 5 times; Wash 5 times with sterile water; then soak with 0.1% mercuric chloride (containing 0.05% Tween-20) for 20 minutes, and finally wash 5 times with sterile water to complete seed disinfection. The sterilized seeds were inoculated into 1.5 mL centrifuge tubes containing callus induction medium, 1 seed per tube. Cultured in a dark environment at 24±2°C, after 1 month, embryogenic callus with bright yellow color, firm texture and...

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Abstract

The invention relates to the technical field of plant tissue culture, in particular to a method for culturing phyllostachys edulis protoplast and inducing cell wall regeneration and application of the method. The protoplast derived from the phyllostachys edulis embryogenic callus is cultured and induced to regenerate the complete cell wall, the regeneration effect is excellent, the success rate is high, and meanwhile, the regeneration result has high repeatability. In addition, the protoplast and cell wall regeneration process can be monitored by the method; a feasible model is provided for researching cell wall synthesis and regulation control mechanisms of phyllostachys edulis and other species, material improvement, biomass energy and other fields; theoretical guidance is provided for improving the quality and yield of bamboo cell walls; and the method has profound influence and long-term significance on the construction and development of the bamboo industry.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for cultivating moso bamboo protoplasts and inducing cell wall regeneration and an application thereof. Background technique [0002] With the rapid development of social economy, the human demand for bamboo products has increased sharply. In recent years, the slow progress of research work on the improvement of bamboo material properties and the selection and breeding of excellent bamboo species has become a key factor restricting the development of bamboo industry. [0003] Plant cell wall is a layer of thick-walled structure existing in the periphery of plant cells. It consists of three parts: intercellular layer, primary wall, and secondary wall. The main component is polysaccharides. The cell wall is mainly involved in maintaining a certain shape of the cell, enhancing the mechanical strength of the cell, and is also related to the physiological activi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
CPCC12N5/04
Inventor 卢存福耿新陈玉珍王晓静
Owner BEIJING FORESTRY UNIVERSITY
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