Application of SNP molecular marker in identification of high-reproductive-performance dairy cows and assisted breeding

A technology of molecular markers and reproductive performance, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of less research on genetic mechanism and the urgent need to strengthen the development of molecular markers, so as to improve the reproductive efficiency , reduce feeding costs and improve breeding efficiency

Active Publication Date: 2021-11-23
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the genetic mechanism of the bovine pregnancy cycle, and the development of related molecular markers needs to be strengthened urgently

Method used

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  • Application of SNP molecular marker in identification of high-reproductive-performance dairy cows and assisted breeding
  • Application of SNP molecular marker in identification of high-reproductive-performance dairy cows and assisted breeding
  • Application of SNP molecular marker in identification of high-reproductive-performance dairy cows and assisted breeding

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Embodiment 1

[0042] The 7th exon of the SIGLEC12 gene affecting the gestation cycle of dairy cows and its molecular markers and applications of the present invention will be described in detail below.

[0043] The present invention affects the 7th exon of the SIGLEC12 gene of the dairy cow gestation cycle and its molecular marker and application, and its specific scheme is:

[0044] 1. Screening SIGLEC12 gene as a candidate gene for dairy cow gestation cycle

[0045] (1) Blood or hair follicle samples were collected from 2,709 Holstein cows in 11 large-scale farms

[0046] Holstein cattle samples were collected in 11 pastures across the country, and the location of the pastures and the number of samples collected in each pasture are shown in Table 1.

[0047] Table 1. Location of pastures where samples were collected and number of samples collected

[0048] The location of the ranch Number of samples The location of the ranch Number of samples Jinchang, Gansu 294 ...

Embodiment 2

[0067] The Sanger sequencing method was used to detect the genotype and allele frequency distribution of the 3 SNP sites of the 7th exon of SIGLEC12 in the Holstein cattle population. The results are shown in Table 2, including the Holstein cattle. The test results showed that there were two genotypes in the Holstein cow population in my country; among all the detected populations, the frequency of GAT / GAT haplotype combination was 97.8%, the frequency of TGC / GAT haplotype combination was 2.2%, and the frequency of GAT haplotype Type is dominant haplotype (see Table 2).

[0068] Table 2 Distribution results of bovine SIGLEC12 gene in Holstein cattle population

[0069]

[0070] Table 2 shows that the above-mentioned group materials are Holstein cattle groups in my country, among which: group 1 is a Holstein cattle group in a ranch in Gansu, group 2 is a Holstein cattle group in a ranch in Hebei, and group 3 is a Holstein cattle group in a ranch in Inner Mongolia Cattle herd...

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Abstract

The invention relates to application of an SNP molecular marker in identification of high-reproductive-performance dairy cows and assisted breeding, and belongs to the technical field of biochemical genetic breeding. Specifically, application of an SIGLEC12 gene as a marker gene of a dairy cow gestation period is provided through a cow 50K SNP chip and a whole genome correlation analysis method, a group of haplotype information of a seventh exon of the SIGLEC12 gene is provided through a molecular biology correlation technology, and the SIGLEC12 gene can be used for selecting dairy cow individuals with dominant gestation period genotypes. Breeding enterprises can improve the frequency of the dominant genotypes of the SIGLEC12 gene gestation period in dairy cow groups by selecting the dominant genotype individuals for seed reservation, so that the gestation period of the dairy cow groups is optimized, the breeding efficiency of the dairy cows is improved, the breeding cost is reduced, and the economic benefit is increased. The invention provides a new method for accelerating the genetic improvement of the gestation period of the dairy cows.

Description

technical field [0001] The invention belongs to the technical field of dairy cow breeding markers, and in particular relates to a SNP molecular marker, a primer sequence for detecting the SNP molecular marker and a kit containing the primer sequence, and also provides the SNP molecular marker, primer and kit in Identification of high reproductive performance dairy cows and applications in assisted breeding. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] With the growth of the world population, the Food and Agriculture Organization of the United Nations (FAO) predicts that the global demand for milk for human consumption will increase by 50% by 2050. For decades, the dai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113Y02P60/87
Inventor 王金鹏黄金明鞠志花杨春红高运东刘文浩肖遥张亚冉魏晓超高亚平姜强王秀革王玲玲赵秀新张元沛周萌
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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