A molecular marker of nfat5 gene related to growth traits of goat and its application

A technology of growth traits and molecular markers, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems that are not available, affect the number of litters, etc., and achieve the effect of rapid detection method

Active Publication Date: 2022-06-03
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that NFAT5 can significantly promote the proliferation of mouse follicular granulosa cells, indicating that it may regulate the development of follicles, thereby affecting the number of litters
So far, there is no relevant report on the study of goat NFAT5 gene as a molecular marker for goat growth traits

Method used

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  • A molecular marker of nfat5 gene related to growth traits of goat and its application
  • A molecular marker of nfat5 gene related to growth traits of goat and its application
  • A molecular marker of nfat5 gene related to growth traits of goat and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Acquisition of goat NFAT5 gene SNP detection fragment and establishment of detection method

[0024] 1. Extraction of goat genomic DNA

[0025] The test goat breeds of the present invention are Macheng black goat, Boer goat and black-headed sheep (a hybrid of Boer goat and Macheng black goat), and the samples are all from the sheep farm of the Animal Husbandry and Veterinary Research Institute of Hubei Academy of Agricultural Sciences. Goat genomic DNA was extracted using the blood genomic DNA extraction kit (Item No.: DP348) produced by Beijing Tiangen Biochemical Technology Co., Ltd. The specific steps were referred to the kit instructions. The extracted DNA was tested for concentration and quality, and stored at -20°C for later use.

[0026] 2. Acquisition of goat NFAT5 gene SNP genetic marker detection fragment

[0027] (1) PCR amplification

[0028] A pair of primers were designed according to the SNP genetic marker detection sequence (shown in SEQ ID ...

Embodiment 2

[0038] Example 2 Detection of polymorphism distribution of molecular markers prepared by the present invention in goat population

[0039] In this example, the polymorphism of the c.-454C>G site in the 5' flanking sequence of the goat NFAT5 gene was detected in the populations of Boer goat, black-headed sheep and Macheng black goat respectively, and the detection results are shown in Table 1.

[0040] Table 1 - Genotype frequency and allele frequency of goat c.-454C>G locus in different goat populations

[0041]

[0042] From the results in Table 1, it can be seen that the c.-454C>G locus has three genotypes in Boer goats and black-headed sheep, except for the absence of GG genotype in Macheng black goats, among which the genotype is homozygous Predominantly, the GG genotype was present in a lower proportion. The dominant alleles of the Boer goat, black-headed sheep and Macheng black goat populations were consistent, and the c.-454C>G locus was the dominant allele in the t...

Embodiment 3

[0043] Example 3 Association analysis and application of goat NFAT5 c.-454C>G molecular markers prepared by the present invention and one-year-old growth traits

[0044] In order to determine whether the c.-454C>G site in the 5' flanking sequence of the goat NFAT5 gene is related to the difference in the growth traits of black-headed sheep, the method established in Example 1 was used for polymorphism detection, and the c.-454C in the goat NFAT5 gene was analyzed. >Correlation of three genotypes of G locus with one-year-old growth traits in goats. SAS statistical software (SAS Institute Inc, Version 9.1) GLM program was used to analyze the association between c.-454C>G locus genotype and one-year-old growth traits, and a significant test was performed. The model used was:

[0045] Y ikjlm =μ+G i +F k +A j +S l +P m +e ikjlm

[0046] Y is the phenotypic value of the trait, μ is the average value of a single trait, G i is the genotype effect, F k is the field fixed eff...

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Abstract

The invention provides a NFAT5 gene molecular marker related to goat growth traits and its application. The molecular marker is located in the 5' flanking sequence of the goat NFAT5 gene; the nucleotide sequence of the molecular marker is shown in the sequence table SEQ ID NO As shown in .1, the sequence length is 392bp, and the sequence includes part of the 5' flanking sequence (-1~-238bp) and part of the 5' untranslated region sequence (+1~+154bp), and the 222bp in the sequence There is a C>G base mutation, and the mutation is named c.-454C>G; the present invention also provides a SNP detection kit associated with goat growth traits, including: as shown in SEQ ID NO.2-3 The primer pair; SNaPshot single base extension primer shown in SEQ ID NO.4. The invention discovers new molecular markers associated with goat growth traits, realizes early selection of goat growth traits, and has a rapid and accurate detection method, which is not affected by breeding environment conditions.

Description

technical field [0001] The invention relates to the technical field of goat molecular marker screening, in particular to an NFAT5 gene molecular marker related to goat growth traits and its application. Background technique [0002] Goat breeding in my country has a long history and is a common source of meat for residents. In recent years, the scale of global goat breeding has continued to expand, especially China is a big goat breeding country. With the continuous growth of consumers' requirements for healthy diets, the proportion of mutton in meat consumption is increasing due to its healthy and safe nutritional properties. Growth traits including body weight and size are the most important economic traits in goat production. Therefore, it is very important to study how to improve the growth traits of goats through breeding for goat industry and goat breeding research. [0003] Single nucleotide polymorphism (SNP) refers to the polymorphism of the DNA sequence caused b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2533/101
Inventor 陶虎陈明新刘洋张年熊琪杨娟李晓锋索效军杨前平田宏张鹤山熊军波张凤
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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