Breed selection method for improving mastitis resistance of dairy cow and use thereof

A technique for mastitis and dairy cows, applied in the field of animal genetics and breeding, can solve the problems of resistance to mastitis in dairy cows without functional SNPs of A2M gene, and achieve the effects of providing accuracy and reliability, reducing economic losses, and increasing resistance

Inactive Publication Date: 2014-12-10
DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports on the existence of alternative splicing and functional SNPs that cause alternative splicing in the A2M gene of cattle, and there are no reports on functional SNPs of the A2M gene and resistance to mastitis in dairy cows

Method used

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  • Breed selection method for improving mastitis resistance of dairy cow and use thereof
  • Breed selection method for improving mastitis resistance of dairy cow and use thereof
  • Breed selection method for improving mastitis resistance of dairy cow and use thereof

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Embodiment Construction

[0055] A realization method of the present invention for improving the anti-mastitis ability of dairy cows and its application are described in detail below.

[0056] The first object of the present invention is to provide a method for cow mastitis susceptibility / resistant A2M candidate gene by real-time fluorescence quantitative PCR (RT-qPCR) and isotope labeling relative and absolute quantification (iTRAQ); by RT-PCR and cloning Sequencing newly discovered two kinds of alternative splicing, and at the same time detected two SNPs of the gene through direct gene sequencing technology, and used ESEfinder3.0 software to analyze the relationship between the newly discovered two kinds of alternative splicing and SNPs, and simultaneously Score (SCS) and A2M genotype were correlated, and the two SNPs loci were found to be functional loci. Selecting individuals with favorable alleles for breeding can improve the anti-mastitis ability of dairy cows, and increase milk production and mi...

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Abstract

The invention provides a breed selection method for improving mastitis resistance of a dairy cow and a use thereof, and belongs to the technical field of animal genetic breeding. The breed selection method comprises the following steps of 1, providing a dairy cow mastitis-susceptible / resistant alpha-2 macroglobulin (A2M) candidate gene by real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) and isobaric tags for relative and absolute quantitation (iTRAQ), 2, analyzing alternative splicing by reverse transcription-polymerase chain reaction (RT-PCR) and cloning sequencing, and simultaneously, detecting single nucleotide polymorphism (SNPs) of the dairy cow mastitis-susceptible / resistant A2M candidate gene by a gene direct sequencing technology, and 3, analyzing the relationship between the alternative splicing and the SNPs by software, simultaneously, carrying out association analysis of a somatic cell score (SCS) and an A2M genetype, determining that a SNPs locus is a functional locus, and selecting a favorable allele individuals as a mastitis-resistant dairy cow breed. Through the breed selection method as a genetic breeding technology, mastitis resistance of a dairy cow is improved; a drug use amount is reduced; milk quality and safety are improved; and an economic loss is reduced.

Description

technical field [0001] The invention relates to the technical field of animal genetics and breeding, in particular to a method for improving the anti-mastitis ability of dairy cows and its application. Background technique [0002] In general, mastitis is one of the most common and complicated diseases in dairy cattle breeding, and it is also the disease that causes the largest loss in dairy farming. At present, there are about 264.57 million dairy cows in the world (FAO, 2010), and about 1 / 3 of dairy cows suffer from mastitis every year. It is estimated that about 95% of cases are caused by Staph.aureus, Strep.uberis and E.coli infections, and the annual loss due to mastitis is as high as 35 billion US dollars ( Wellenberg et al., 2002). The research on the pathogenesis and prevention of cow mastitis has a history of more than 100 years, and has made remarkable progress. However, dairy cow mastitis is a very complex disease. Although many countries have implemented compr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11A01K67/027
Inventor 黄金明王秀革袁金铎王长法鞠志花李秋玲齐超张燕李荣岭李建斌侯明海仲跻峰
Owner DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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