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Marker for evaluating tumor tissue CD47 expression level in real time, and application of marker

A technology of expression level and tumor tissue, applied in the field of tumor immunotherapy evaluation, can solve the problems of inability to realize real-time detection and uneven evaluation level, and achieve the effect of high promotion and application value, high real-time and accuracy, and high acceptance.

Inactive Publication Date: 2021-12-07
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main way to detect CD47 is immunohistochemical method, but there are the following problems: 1. "Immunohistochemical method" is used to visually detect the expression of CD47 protein in tumor tissue, but real-time detection cannot be realized; 2. The diversity of immunohistochemical antibodies, Inhomogeneous assessment levels

Method used

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  • Marker for evaluating tumor tissue CD47 expression level in real time, and application of marker
  • Marker for evaluating tumor tissue CD47 expression level in real time, and application of marker

Examples

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Embodiment 1

[0027] This example will explore the relationship between exosome ZEB1 and cervical cancer tissue CD47 from the following two parts.

[0028] Part 1: Hypoxic cervical cancer cells highly express ZEB1 and promote the transcriptional regulation of CD47.

[0029] Immunofluorescence, Western Blot, and fluorescent quantitative PCR were used to detect the expression levels of ZEB1 and CD47 in cervical cancer cell lines (SiHa and C33a) cultured under normoxic and hypoxic conditions. Cells highly express ZEB1 and CD47 ( figure 1 A).

[0030] Using lentivirus to knock down or overexpress the expression level of cervical cancer cell line ZEB1, the expression level of CD47 was also decreased or increased ( figure 1 B and figure 1 C). The double-luciferase results confirmed that ZEB1 is an upstream transcription factor of CD47, and transcriptionally up-regulates the expression of CD47 ( figure 1 D).

[0031] The second part: The relative expression of plasma exosome ZEB1 in patients...

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Abstract

The invention belongs to the field of tumor immunotherapy evaluation, and particularly relates to a marker for evaluating a tumor tissue CD47 expression level in real time, and application of the marker. Based on in-vitro experimental research, ZEB1 is found to be an important transcription factor for regulating and controlling expression of cervical cancer cells CD47, and a phenomenon that the ZEB1 is enriched in exosomes in hypoxia cervical cancer cells is found for the first time; the expression quantity of the blood plasma exosome ZEB1 of the patient is further verified to be in positive correlation with the expression quantity of the tumor tissue CD47 in a cervical cancer clinical sample, and the expression quantity of the CD47 in the cervical cancer tissue can be reflected by detecting the expression level of the blood plasma exosome ZEB1 of the cervical cancer patient, so that important guiding significance is performed in cervical cancer CD47 immunotherapy evaluation.

Description

technical field [0001] The invention belongs to the field of tumor immunotherapy evaluation, and relates to a marker for real-time evaluation of CD47 expression level in tumor tissue and its application. Background technique [0002] With the in-depth study of innate immune checkpoints, the field of tumor immunology has ushered in a new era of cancer treatment, and inhibitory immune checkpoint blockade is one of the most important advances in anticancer therapy. As a novel immune checkpoint molecule, CD47 plays an important role in immune response and tumor immune escape. CD47 binds to the signal regulatory protein α (SIRPα) on the surface of macrophages and initiates the "don't eat me" inhibitory signal, so that tumor cells can avoid the phagocytosis of macrophages and affect the activation of specific tumor-killing T cells, mediating lead to tumor progression. CD47-SIRPα axis immune blockers are one of the most promising new targets in tumor immunology in the past decade...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/68G01N33/574
CPCC12Q1/6886G01N33/68G01N33/57411G01N33/57484C12Q2600/158C12Q2600/106G01N2333/4703G01N2800/52G01N2333/70503
Inventor 王薇陈晓静梁莉吴砂郭楚鸿
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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