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Kit for detecting laryngeal squamous cell carcinoma radiotherapy sensitivity related gene ATM and ATR gene mutation and application of kit

A technology of laryngeal squamous cell carcinoma and kit, which is applied in the field of kits for detection of mutations of genes related to radiotherapy sensitivity of laryngeal squamous cell carcinoma, ATM and ATR, can solve the problems of high price, achieve high accuracy, avoid waste of medical resources, and improve sensitivity and specific effects

Active Publication Date: 2021-12-07
EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV
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Problems solved by technology

At present, the detection of ATM and ATR gene mutations in laryngeal squamous cell carcinoma is not a routine clinical item, and the price of single detection is expensive. The development of new detection kits has great research value

Method used

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  • Kit for detecting laryngeal squamous cell carcinoma radiotherapy sensitivity related gene ATM and ATR gene mutation and application of kit
  • Kit for detecting laryngeal squamous cell carcinoma radiotherapy sensitivity related gene ATM and ATR gene mutation and application of kit

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Embodiment 1

[0037] Example 1 Construction of scoring model for ATM / ATR mutation in laryngeal squamous cell carcinoma

[0038] 1. Method

[0039] We first obtained the data of 111 samples of laryngeal squamous cell carcinoma from the TCGA database, divided the samples into mutation group (n=11) and wild group (n=100) according to whether there was ATM or ATR gene mutation, and then calculated and screened out 184 differential gene. By performing LASSO regression analysis on the expression data [log2(FPKM+1) form] of the above 184 differential genes in 111 samples, we obtained 12 genes significantly related to ATM / ATR gene mutations: VSTM2L, TSPAN8, RDH10, PRODH, PITX2, ODC1, MASP1, KLHDC8A, GPRC5D, CTAG2, CCL26, ABCA13.

[0040] Construct scoring model by binary Logistic regression (-0.7519×VSTM2L)+(-0.7602×TSPAN8)+(0.1142×RDH10)+(0.1816×PRODH)+(0.3597×PITX2)+(0.0034×ODC1)+(-0.3224× MASP1)+(0.4007*KLHDC8A)+(0.0877*GPRC5D)+(0.1375*CTAG2)+(-0.9765*CCL26)+(0.4486*ABCA13).

[0041] We calc...

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Abstract

The invention relates to the technical field of molecular diagnosis, in particular to a kit for detecting laryngeal squamous cell carcinoma radiotherapy sensitivity related gene ATM and ATR gene mutation and an application of the kit. The kit consists of reagents for detecting expression quantities of VSTM2L, TSPAN8, RDH10, PRODH, PITX2, ODC1, MASP1, KLHDC8A, GPRC5D, CTAG2, CCL26 and ABCA13. According to the method, genes related to ATM and ATR mutation are screened by applying RNA sequencing, LASSO and binary classification Logistic regression, a Score is constructed, a threshold value corresponding to the Score in the laryngeal squamous cell carcinoma is obtained through ROC analysis, and the method can be used for predicting ATM and ATR gene mutation in the laryngeal squamous cell carcinoma. The specific gene mutation related to the laryngeal squamous cell carcinoma radiotherapy sensitivity is predicted by utilizing the expression quantity of a gene composition, and verification shows that the detection method has the advantages of simplicity and convenience in operation, high accuracy and good specificity, and has a very good application prospect.

Description

technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a kit for detecting mutations of genes ATM and ATR related to radiosensitivity of laryngeal squamous cell carcinoma and its application. Background technique [0002] Laryngeal cancer is a high-incidence tumor of the head and neck, and squamous cell carcinoma is its most common pathological type. Radiotherapy is one of the main treatment methods for laryngeal squamous cell carcinoma, and it has a very good therapeutic effect on most patients with laryngeal squamous cell carcinoma. However, in some patients with throat cancer, radiation therapy does not respond well. If the radiosensitivity of a patient's tumor can be judged as early as possible, it can be accurately judged whether the patient is suitable for radiotherapy, thereby guiding clinical practice, and greatly improving the prognosis of patients, reducing unnecessary costs for patients and waste of medical re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574G01N33/68G16B20/50
CPCC12Q1/6886G01N33/57407G01N33/6893G16B20/50C12Q2600/156C12Q2600/158
Inventor 燕丽王孝深朱奕宋新貌宋小乐杨钢
Owner EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV
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