Reagent, kit and method for detecting mutations of genes related to lung squamous cell carcinoma anti-oxidative stress driving pathway

A technology related to anti-oxidative stress and pathways, applied in biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effects of high accuracy, economical benefits, and avoiding waste of medical resources

Pending Publication Date: 2021-12-03
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Application Information

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Problems solved by technology

There is no report on the kit for detecting gene mutations related to the antioxidant stress-driven pathway in lung squamous cell carcinoma

Method used

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  • Reagent, kit and method for detecting mutations of genes related to lung squamous cell carcinoma anti-oxidative stress driving pathway
  • Reagent, kit and method for detecting mutations of genes related to lung squamous cell carcinoma anti-oxidative stress driving pathway
  • Reagent, kit and method for detecting mutations of genes related to lung squamous cell carcinoma anti-oxidative stress driving pathway

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Experimental program
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Effect test

Embodiment 1

[0031] Construction of scoring model for gene mutations related to antioxidant stress-driven pathways in lung squamous cell carcinoma

[0032] Methods: Firstly, the data of 484 lung squamous cell carcinoma samples were obtained from the TCGA database, and the samples were divided into mutation groups (n=143) and In the wild group (n=341), the expression data of the transcriptome RNA expression profiles of the coding proteins of 484 samples were expressed in [log 2 (FPKM+1) format] Lasso regression analysis was performed to obtain genes significantly related to the mutation status of genes related to the antioxidant stress pathway, and finally 12 genes were selected: SRXN1, TRIM16L, CYP4F3, DIRAS2, C18orf65, NR0B1, LURAP1 , ABCC2, FECH, NQO1, BLOC1S4, TXNRD1.

[0033]In addition, continue to construct the scoring model RiskScore=(0.2817×SRXN1)+(0.0219×TRIM16L)+(0.0361×CYP4F3)+(0.012×DIRAS2)+(0.0229×C18orf65)+(0.0497×NR0B1)+( -0.2434×LURAP1)+(0.0434×ABCC2)+(0.106×FECH)+(0.0532...

Embodiment 2

[0040] Example 2: Effect Verification

[0041] RNA sequencing was performed on 30 cases of lung squamous cell carcinoma samples collected from the Department of Thoracic Surgery, Zhongshan Hospital Affiliated to Fudan University, and the mutations of genes related to the antioxidant stress pathway (KEAP1, NFE2L2, CUL3) were detected. The RNA expression data were brought into the prediction model to obtain each For the RiskScore score of the samples, those whose scores are higher than the cutoff value of 5.014 are the mutant group, and those whose scores are lower than the cutoff value of 5.014 are the wild group.

[0042] The experimental results are shown in Table 1:

[0043] Table 1 Experimental data

[0044] mutant group wild group total Score ≥ 5.014 7 2 9 Score 1 20 21 total 8 22 30

[0045] In summary, the sensitivity of the prediction model in the case cohort of Zhongshan Hospital is 0.875, and the specificity is 0.910.

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Abstract

The invention provides a reagent, kit and method for detecting mutations of genes related to a lung squamous cell carcinoma anti-oxidative stress driving pathway. Genes related to the anti-oxidative stress pathway are screened by applying RNA sequencing and Lasso algorithm characteristics, a model is constructed by constructing a score RiskScore and applying dichotomy logistic regression, the optimal cut-off value of the score RiskScore in the lung squamous cell carcinoma is obtained through an ROC curve, and the reagent, the kit and the method can be used for predicting the mutation of the genes (KEAP1, NFE2L2 and CUL3) related to the anti-oxidative stress pathway in the lung squamous cell carcinoma. The mutation of the genes related to a specific driving pathway of the lung squamous cell carcinoma is predicted by utilizing the expression quantity obtained by combining the genes, and TCGA database verification shows that the method has the advantages of high sensitivity and good specificity, can effectively guide individualized treatment of patients with the lung squamous cell carcinoma, adds more important hopes for precise treatment and individualized treatment, and improves the clinical benefit.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis, and in particular relates to a reagent, a kit and a method for detecting gene mutations related to an anti-oxidative stress-driven pathway of lung squamous cell carcinoma. Background technique [0002] According to the latest 2020 global cancer statistics report, the morbidity and mortality of lung cancer are at the forefront of all tumors in 185 countries around the world, and the number of deaths from lung cancer accounts for nearly 20% of all cancer deaths. As the second most common type of lung cancer, squamous cell carcinoma of the lung is not to be underestimated. According to previous epidemiological data, the incidence and mortality of squamous cell carcinoma of the lung rank first. Although a variety of mutation sites and targeted drugs for various mutations have emerged in an endless stream, the treatment methods for lung adenocarcinoma patients have been enriched, and the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/106C12Q2600/156C12Q2600/158
Inventor 金星张欢单光耀梁嘉琪胡正阳詹成王群
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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