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Method for improving beef cattle meat production performance by using MYBPC1 gene

A beef cattle and gene technology, applied in the field of molecular biology, can solve the problems of poor fat deposition, slow growth and low meat production of Qinchuan cattle, and achieve the effect of improving meat production performance

Pending Publication Date: 2021-12-07
INNER MONGOLIA UNIVERSITY +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition.

Method used

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  • Method for improving beef cattle meat production performance by using MYBPC1 gene
  • Method for improving beef cattle meat production performance by using MYBPC1 gene
  • Method for improving beef cattle meat production performance by using MYBPC1 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Obtaining SNP Detection Fragments of Qinchuan Cattle and Chinese Simmental Cattle MYBPC1 Gene and Establishment of Polymorphism Site Detection Method

[0033] 1. Extraction of Genomic DNA from Qinchuan Cattle and Chinese Simmental Cattle

[0034] The test cattle of the present invention are 350 Qinchuan adult cows (age 18-24 months old, at least three generations unrelated) of the National Beef Cattle Improvement Center of Northwest Agriculture and Forestry University, and 260 Chinese Ximen cows of Chifeng Shengquan Ecological Animal Husbandry Co., Ltd. Tahr cattle (age 14-18 months). The genomic DNA of Qinchuan cattle and Chinese Simmental cattle was extracted using the blood genomic DNA extraction kit produced by Beijing Tiangen Biochemical Technology Co., Ltd., and the specific steps refer to the kit instructions. Evaluate the quality and quantity of the extracted DNA, evaluate it by agarose gel electrophoresis, and store it at -40°C for future use.

[00...

Embodiment 2

[0049] Example 2 Detection of polymorphic distribution of molecular markers in Qinchuan cattle, Chinese Simmental cattle and other local beef cattle breeds

[0050] The present invention detects the genetic diversity of the g.65393435A>G site of the MYBPC1 gene in 7 cattle populations, and the detection results are shown in Table 3.

[0051] Table 3 The genotype frequency and allele frequency of g.65393435A>G locus of MYBPC1 gene in cattle population

[0052]

[0053] Note: QC, Qinchuan cattle; LX, Luxi cattle; MGC, Mongolian cattle group (Inner Mongolia Autonomous Region, China); MGG, Mongolian cattle group (Mongolia); WL, Wuling cattle; LL, Longlin cattle; CS, China Simmental.

[0054] From the results in Table 3, it can be seen that the genotype frequencies of the g.65393435A>G site of the Chinese Qinchuan cattle MYBPC1 gene are: GG (0.831), GA (0.151), AA (0.017), and the G allele frequency (0.907) is higher than The A allele frequency (0.093); the G allele frequency ...

Embodiment 3

[0055] Example 3 Correlation Analysis and Application of Molecular Markers and Meat Performance of Qinchuan Cattle and Chinese Simmental Cattle

[0056] In the present invention, SPSS 19.0 is used to analyze the correlation analysis between the g.65393435A>G site of the MYBPC1 gene of Qinchuan cattle and Chinese Simmental cattle and the meat production performance, and the general linear model is constructed as follows:

[0057] Y ijklm =μ+G i +A j +F k +S l +S m +e ijklm ;

[0058] Among them, Yijklm is the observed value of the trait, μ is the average value of the trait, G i is the genotype effect, A j is the fixed effect caused by age, F k is the pasture environmental effect, S l is the gender effect, S m is the family effect, e ijklm is a random error.

[0059] Correlation analysis of MYBPC1 gene g.65393435A>G locus and meat production performance in Qinchuan cattle and Chinese Simmental cattle, the AA genotype individuals are less than 10, no statistics, GG a...

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to a method for improving beef cattle meat production performance by using a g.65393435A>G site of an MYBPC1 gene. The present invention provides a method for improving beef cattle meat production performance by using a g.65393435A>G site of an MYBPC1 gene. The method comprises the following steps: detecting whether mutation of the g.65393435A>G site exists in the MYBPC1 gene in a beef cattle genome to be detected or not; selecting a beef cattle individual of which the genotype is GG or GA, and carrying out artificial insemination and native mating. The molecular marker is used as a reliable marker for the meat production performance of the Qinchuan cattle and the Chinese Simmental cattle, and a technical system for detecting the genotype through DNA is established, so that the selection intensity and the breeding accuracy and efficiency are improved, and the meat production performance of the Qinchuan cattle and the Chinese Simmental cattle is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for improving the meat production performance of beef cattle by utilizing the g.65393435A>G site of the MYBPC1 gene. Background technique [0002] Qinchuan cattle are named after the "Eight Hundred Li Qinchuan" located in Guanzhong, Shaanxi. Appropriate pattern and other characteristics. Qinchuan cattle breeding has become a pillar industry for farmers in Shaanxi, Gansu, Ningxia and other provinces to increase their income. However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition. [0003] Simmental cattle are native to Switzerland. In 1990, the cattle and sheep breeding base of Shandong Provincial Animal Husbandry Bureau introduced this breed. Simmental cattle are not purebred beef cattle, but due to their high milk production, the meat produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686
CPCC12Q1/6888C12Q1/686C12Q2600/124C12Q2600/156C12Q2565/125Y02P60/87
Inventor 佟彬盛聪成功昝林森青格乐图殷志成
Owner INNER MONGOLIA UNIVERSITY
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