Alcohol-soluble homogeneous polysaccharide and anti-depression application thereof

An alcohol-soluble and uniform technology, applied in the direction of polysaccharide/gum-containing food ingredients, applications, and resistance to vector-borne diseases, etc., to achieve strong protective effects, improve hippocampal damage, and improve depression-like behaviors

Active Publication Date: 2021-12-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this extraction method only focuses on the water-soluble polysaccharides in th

Method used

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  • Alcohol-soluble homogeneous polysaccharide and anti-depression application thereof
  • Alcohol-soluble homogeneous polysaccharide and anti-depression application thereof
  • Alcohol-soluble homogeneous polysaccharide and anti-depression application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040]This example relates to the separation and preparation of a natural plant alcohol-soluble homogeneous polysaccharide (ASP), specifically comprising: taking 500 g of dried flowers of Dendrobium officinale Kimura et Migo, pulverizing them, the ratio of solid to liquid is 1:10, and the temperature is set to 110 ℃, heated and refluxed for three times, and the filtrate was concentrated to a thick paste to obtain an aqueous extract; the aqueous extract was redissolved in water, and loaded onto AB-8 macroporous resin at different concentrations (0%, 10%, v / v) Eluted with ethanol, the combined fractions were concentrated and dried under reduced pressure. Then it was fully dissolved in water, precipitated by 4 times the volume of absolute ethanol, centrifuged, the supernatant was collected and concentrated, loaded on AB-8 macroporous resin again, eluted with water, and the sugar content was determined by the phenol-sulfuric acid method, combined The eluate containing sugar (the e...

Embodiment 2

[0043] This embodiment relates to the structural characterization of Dendrobium officinale flower alcohol-soluble homogeneous polysaccharide (ASP), including:

[0044] Step 1) Determination of total sugar content: the total sugar content of ASP was determined to be 85.33% by phenol sulfuric acid method.

[0045] Step 2) ultraviolet spectrum analysis: measure the ultraviolet absorption of ASP aqueous solution with scanning mode (200~500nm) by spectrophotometer; ASP does not have absorption peak at 260 and 280nm place in UV-vis spectrum ( image 3 ), indicating that it does not contain protein and nucleic acid.

[0046] Step 3) Homogeneity and Molecular Weight Determination: ASP is prepared into a 0.5 mg / mL solution with ultrapure water, and the homogeneity test is performed by high-performance liquid gel permeation chromatography. The chromatographic column is a TSK-GEL G4000 SWXL gel column, and the column temperature is set The temperature is 37°C, the detector is Agilent 12...

Embodiment 3

[0066] The alcohol-soluble homogeneous polysaccharide of Dendrobium candidum prepared as above in the present embodiment was used to observe its acute antidepressant effect in a mouse model of behavioral despair, specifically as follows:

[0067] Experimental animals: male ICR mice, SPF grade, 18-25g.

[0068] Experimental drugs: blank group (Control) was given 10mL / kg.bw of normal saline; positive drug group (Imipramine) was given 15mg / kg.bw of imipramine; ASP low-dose group (ASP(L)) was given 94mg / kg. ASP in bw; ASP high dose group (ASP(H)) was given 234 mg / kg.bw of ASP.

[0069] Experimental method: The mice were randomly divided into 4 groups, 12 in each group. After one week of adaptive feeding, the corresponding drugs were administered orally every day for one week. On the 8th, 9th, and 10th days, the open field test (OFT), Tail suspension test (TST), forced swim (FST) test.

[0070] Open field test: The open field test was carried out on the 8th day after the administ...

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Abstract

The invention relates to an alcohol-soluble homogeneous polysaccharide and an anti-depression application thereof, in particular to a dendrobium officinale flower alcohol-soluble homogeneous polysaccharide, a preparation method thereof and an application of the dendrobium officinale flower alcohol-soluble homogeneous polysaccharide in preparation of an anti-depression drug. The alcohol-soluble homogeneous polysaccharide is obtained by purifying separated components of a natural plant aqueous extract for multiple times, and the alcohol-soluble homogeneous polysaccharide is named as ASP (Alcohol-soluble polysaccharide); and in-vivo experiments prove that the alcohol-soluble homogeneous polysaccharide has remarkable anti-depression activity, the action effect of the alcohol-soluble homogeneous polysaccharide is equivalent to that of fluoxetine serving as an anti-depression marketing drug, and the alcohol-soluble homogeneous polysaccharide can be further developed and prepared into the anti-depression drugs.

Description

technical field [0001] The invention belongs to the field of natural product development and relates to polysaccharides, in particular to a natural alcohol-soluble homogeneous polysaccharide in Dendrobium officinale flowers and its antidepressant application. Background technique [0002] Depression is one of the common mental disorders, characterized by significant and persistent low mood. The World Health Organization (WHO) reports it is now the leading cause of disability worldwide and a significant contributor to the global burden of disease. The drugs currently used to treat depression are mainly neurotransmitter reuptake inhibitors, such as fluoxetine, venlafaxine, imipramine, etc., but most of these drugs have symptoms such as sweating, dizziness, and gastrointestinal functional reactions. side effect. Therefore, it is of great significance to find alternative drugs with less side effects from natural plants. [0003] Natural plant polysaccharides have great potent...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P25/24A23L33/125
CPCC08B37/006C08B37/0003A61P25/24A23L33/125A23V2002/00A23V2200/322A23V2250/51Y02A50/30
Inventor 李晓波杨燕彭颖范莉
Owner SHANGHAI JIAO TONG UNIV
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