Porcine embryo culture solution and application

Abstract

The invention provides a porcine embryo culture solution. The culture solution is a PZM-3 culture solution containing the following concentration components: 1-8 g / L of reduced glutathione, 10-50 mg / L of tea polyphenol and 8-15 v / v% of pig follicular fluid. The culture solution is prepared by adding reduced glutathione, tea polyphenol and pig follicular fluid with proper concentration into the existing PZM-3 culture solution. The reduced glutathione and the tea polyphenol can be combined to achieve a synergistic effect, so that the effect of the culture solution on promoting the development of the pig embryos is superior to that of the culture solution which is independently used with the reduced glutathione or the tea polyphenol. And the use of the pig follicular fluid further improves the efficacy of the culture solution. The culture solution provided by the invention can effectively promote the development of the pig in-vitro culture embryo, improve the 4-cell cleavage rate and blastocyst rate of the pig in-vitro culture embryo and the total cell number of the blastocyst, and has a wide application prospect in the in-vitro production of the pig embryo.

Description

technical field [0001] The invention belongs to the technical field of pig breeding, and in particular relates to a pig embryo culture solution and application. Background technique [0002] In recent years, in vitro production of embryos has been widely used in the production of animals with economic value. At the same time, in vitro fertilization (IVF), single sperm injection (ICSI), embryo transfer, embryo segmentation and embryonic stem cell engineering are extremely Greatly promoted the development of animal husbandry. With the development of modern reproductive biotechnology and the gradual improvement of in vitro embryo production technology, the use of in vitro fertilization technology to produce in vitro embryos presents a potential broad application prospect. In vitro production of livestock embryos is an effective way to rapidly expand the number of outstanding populations. Although live offspring have been obtained by in vitro fertilization technology, the in v...

AI Technical Summary

Problems solved by technology

With the rapid development of social economy and animal husbandry and other fields, there is an urgent need to prepare more and better animal embryos in vitro. However, in the actual production process, embryos developed in vitro are blocked at a certain stage, which wastes a lot of resources. , while pig embryos are severely arrested at the 4-cell stage during in vitro development

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