Poplar latex protein MLP gene capable of improving cadmium stress resistance of plants, protein expressed by poplar latex protein MLP gene and application

Abstract

The invention discloses a poplar latex protein MLP gene capable of improving the cadmium stress resistance of a plant and an expressed protein and application thereof, and relates to a gene capable of improving the cadmium stress resistance of the plant and an expressed protein and application thereof. The nucleotide sequence of the poplar latex protein MLP gene capable of improving the cadmium stress resistance of the plant is shown as SEQ ID NO: 1. The amino acid sequence of the poplar latex protein MLP expressed by the gene is shown as SEQ ID NO: 2. The gene is used for improving the cadmium stress resistance of plants. The MLP gene of the transgenic MLP gene plant is overexpressed under the condition of CdCl2 stress, so that the Cd < 2 + > absorption of the transgenic plant is reduced, the POD enzyme activity in an active oxygen scavenging system is increased, and the content of an osmotic regulation substance proline is increased, thereby reducing the damage of CdCl2 stress to the plant.

Description

technical field [0001] The present invention relates to a gene which can improve the ability of plants to resist cadmium stress, its expressed protein and its application. Background technique [0002] Poplar has a wide distribution, strong adaptability and fast growth rate. It is an important afforestation and timber species in my country, and it is also a model plant for the study of forest physiology and genetic engineering. However, in areas where water resources are scarce and salinization is serious, the growth and development of poplar trees are greatly affected. The rapid development of modern molecular biology and its wide application in the research of forest tree genetics and breeding provide a new idea and method for cultivating new tree varieties with high yield, stable yield and strong stress resistance in a relatively short period of time. [0003] Major latex protein (MLP) is a type of protein unique to plants. Since Nessler et al. obtained latex protein fr...

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Problems solved by technology

However, there has been no literature report on the MLP gene of poplar, and there is no report on the relationship between MLP and plant resistance to heavy metal cadmium stress.

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