Anesthesia-free directional embedding method and system for model animals

Abstract

The invention discloses a model animal anesthesia-free directional embedding method and system, the system comprises a zebra fish micro-fluidic chip and a droplet array chip, the zebra fish micro-fluidic chip comprises a micro-fluidic chip substrate and a cover plate; the micro-fluidic chip substrate and the cover plate are connected in a non-permanent combination manner. The micro-fluidic chip can realize the preparation of the zebra fish capsule only by simple cooling, and the obtained zebra fish capsule has the same activity as non-fixed zebra fish, and has high survival rate. The zebra fish anesthesia-free directional embedding medicine screening system is simple to construct, small in size, convenient to use, low in cost and free of assistance of peripheral equipment, the medicine screening experiment dosage is reduced from mL level to mu L level, and high throughput, multiple phenotypes and low consumption in the real sense are achieved.

Description

technical field [0001] The invention belongs to the technical field of embedding of model animals, and in particular relates to a non-anesthesia directional embedding method and system for model animals. Background technique [0002] In related technologies, one of the mainstream means of using zebrafish for drug screening technology is microfluidic chip technology. The microfluidic chip uses continuous reverse water flow, combined with its special microstructure design, to fix zebrafish. The use of fluidic chips and their use in immobilization of model animals has greatly reduced zebrafish damage. However, the microfluidic chip in the related technology requires continuous water supply, which leads to the continuous injection or preparation of a large amount of drug solutions to achieve the rigor of drug screening tests. In the process of drug development, the price of general drugs to be screened is relatively high Expensive or small output, unable to maintain the needs o...

AI Technical Summary

Problems solved by technology

However, the microfluidic chip in the related technology requires continuous water supply, which leads to the continuous injection or preparation of a large amount of drug solutions to achieve the rigor of the drug screening test. In the process of drug development, the price of general drugs to be screened is relatively high Expensive or small output, unable to maintain the needs of large-scale trials for a long time, and even to a certain extent cannot meet the needs of high-throughput use, which poses a huge challenge to the accuracy of drug screening and test cycle, therefore, There is an urgent need to develop new technologies to reduce the amount of drug screening

[0003] Although the traditional agarose embedding and fixation technology can avoid the waste of drugs to a certain extent, this embedding method cannot achieve the precise orientation of the zebrafish, and it is fundamentally unable to meet the precise imaging of specific organs, and the artificial agarose Embedding and fixation is time-consuming and laborious, the consumption rate of model organisms is high, it is difficult to perform high-throughput screening, and the screening efficiency is extremely low

Moreover, even if the zebrafish orientation is successfully adjusted manually, it is easy to cause irreversible damage to the zebrafish when observing a specific organ, and the accuracy of this manual adjustment is also unpredictable, and it is impossible to achieve a real comparison. to request

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