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Method for testing soothing efficacy of cosmetics and raw materials thereof

A testing method and cosmetic technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of lack of national standards and industry standards, and achieve economical and fast, controllable testing process, and standardized testing system. Effect

Pending Publication Date: 2022-04-01
广东悠质检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no national standard or industry standard for the evaluation method of soothing efficacy

Method used

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  • Method for testing soothing efficacy of cosmetics and raw materials thereof
  • Method for testing soothing efficacy of cosmetics and raw materials thereof
  • Method for testing soothing efficacy of cosmetics and raw materials thereof

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Embodiment 1

[0034] The invention provides a cell test method for the soothing effect of cosmetics and raw materials thereof, which uses in vitro cells as a testing means to evaluate the soothing effects of cosmetics and raw materials thereof.

[0035] Wherein, the cell test method is mainly divided into cell subculture, sample cytotoxicity test, and sample NO release inhibition test.

specific Embodiment approach

[0037] 1. Cell culture: the mouse macrophage RAW264.7 cell line was used for testing.

[0038] The cryopreserved mouse macrophage RAW264.7 cells were taken out from the liquid nitrogen tank, and the cells were recovered and subcultured by conventional technical means. The specific resuscitation culture method is as follows: quickly dissolve the frozen cells in a water bath at about 37°C, centrifuge at a low speed of 800-1000rpm for 5-10min, discard the supernatant, resuspend the cells at the bottom with DMEM medium containing 10% bovine serum, transfer to In the T25 cell bottle, place the temperature at 37°C, CO 2 The concentration is 5.0% and cultured for 24-72 hours, and subcultured when the cell confluency reaches over 80%. Subculture method is as follows: Discard the cell culture medium, gently wash once with fresh serum-free medium or PBS, discard, add 5-10ml DMEM medium containing 10% bovine serum, wash the cells at the bottom of the cell flask or Collected by cell scr...

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Abstract

The invention discloses a soothing efficacy test method for cosmetics and raw materials thereof, and aims to provide a test method for evaluating the soothing efficacy of cosmetics by adopting cells as a detection means, the method adopts the cells to replace human body tests, accords with the '3R' principle of experimental animals, and is economical and rapid, controllable in test process and standardized in test system. And large result deviation caused by individual differences of human bodies is avoided. The test method comprises the following steps: culturing a mouse macrophage cell strain in vitro, adding a sample to be tested into cells for testing, firstly testing the cytotoxicity of the sample, then selecting a concentration without cytotoxicity to carry out a nitric oxide (NO) release inhibition test, and analyzing and evaluating the soothing effect of the sample through a statistical means. According to the test method, through multiple times of practical application, a judgment standard of the relieving effect is established. The test method is used for evaluating the soothing efficacy of cosmetics and raw materials thereof, and belongs to the technical field of cosmetics.

Description

technical field [0001] The invention relates to a test method, in particular to a test method for the soothing efficacy of cosmetics and raw materials thereof, and belongs to the technical field of cosmetic test. [0002] technical background [0003] The soothing effect of cosmetics refers to the improvement of skin irritation and other conditions after using cosmetics. Cosmetics with a soothing effect should pass the cosmetic efficacy claim evaluation test method, which can be combined with literature or research data analysis results to evaluate the efficacy claim. According to the requirements of the cosmetic efficacy claim evaluation project, the soothing effect can be evaluated by any of the three methods: human body efficacy evaluation test, consumer use test and laboratory test. However, at present, there is no national standard or industry standard for the evaluation method of soothing efficacy. [0004] Lipopolysaccharide (LPS) is an endotoxin derived from Gram-ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 郭沈涛徐文枫陈媛崔玉矫李适炜
Owner 广东悠质检测技术有限公司
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