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Detection kit for identifying novel duck reovirus and novel goose reovirus and application thereof

A technology of duck reovirus and detection kit, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effects of improving efficiency, simple operation and high sensitivity

Active Publication Date: 2022-06-10
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently reported methods for detecting waterfowl-derived reovirus pathogens mainly include: virus isolation and identification, agar diffusion test, common PCR and fluorescent quantitative PCR, etc. However, there is currently no rapid detection and identification method that can effectively target the new type of duck reovirus and the new type of goose reovirus

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  • Detection kit for identifying novel duck reovirus and novel goose reovirus and application thereof
  • Detection kit for identifying novel duck reovirus and novel goose reovirus and application thereof
  • Detection kit for identifying novel duck reovirus and novel goose reovirus and application thereof

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Embodiment Construction

[0065] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0066] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.

[0067] Design and construction of primers and probes

[0068] The inventors have designed a large number of primers and probes based on the new duck reovirus and the new goose reovirus. Among them, the new duck reovirus NCBI accession number is NO. Reovirus reference Huang Y, Zhang J, Dong J, et al. Isolation and characterization of a newgoose orthoreovirus causing liver and spleen focal necrosis in geese, China [J]. Tr...

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Abstract

The invention discloses a detection kit for identifying a novel duck reovirus and a novel goose reovirus and application thereof. The kit is composed of a probe as shown in SEQ ID NO: 3 and primer pairs as shown in SEQ ID NO: 1-2. The detection method based on the detection kit is high in detection speed and high in throughput, and has good specificity for the novel duck reovirus and the novel goose reovirus, the lowest detection limit for the novel duck reovirus can reach 17.8 copies, and the lowest detection limit for the novel goose reovirus can reach 35.3 copies. The detection method is of great significance to monitoring, prevention and control of the novel duck reovirus and the novel goose reovirus.

Description

technical field [0001] The invention relates to the field of virus detection, in particular to a detection kit for identifying a new type of duck reovirus and a new type of goose reovirus and an application thereof. Background technique [0002] The new duck reovirus disease is caused by the new duck reovirus (NDRV), and its onset is characterized by soft feet, plaque-like hemorrhage and necrosis on the liver and spleen and other organs, and the kidneys are enlarged, bleeding, and yellowish-white on the surface stripes. New duck reovirus disease is an acute, severe, viral infectious disease with high morbidity and mortality. Except for the slightly poor susceptibility of shelduck to the disease, other breeds of ducks can be affected. [0003] The novel goose reovirus (NGRV) was isolated and identified for the first time in 2020 by the inventor's research group from the diseased goose. disease. After sequence and phylogenetic analysis, it can be seen that the homology of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/70C12Q1/686
CPCC12Q1/701C12Q1/686C12Q2563/107
Inventor 董嘉文李林林陈修邓张俊勤廖明陆巧芬孙敏华曹建伟黄允真沈书文吴晓斌
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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