Quick constant temperature control method and control device for PCR (polymerase chain reaction) detector and PCR detector

Abstract

The invention discloses a control method and a control device for rapid constant temperature of a PCR (Polymerase Chain Reaction) detector and the PCR detector, in the control method for rapid constant temperature of the PCR detector, an integral separation method is operated in the whole process, and when the output quantity is 0 lt; and utt; when umax is greater than or equal to umax, integral is introduced, PID control is adopted, and when ut is greater than or equal to umax or ut is less than or equal to 0, PD control is adopted. In the temperature changing stage, a time optimal PID control algorithm and a traditional PID control algorithm are adopted for heating or cooling; a traditional PID control algorithm is adopted in the constant temperature stage; at a variable-temperature and constant-temperature switching time point, a temperature fast-setting control algorithm and a self-adaptive control algorithm are adopted; the method has the advantages of being accurate in temperature control, excellent in temperature change benefit, fast in temperature change, fast in constant temperature and small in overshoot, it can be guaranteed that the target value can be reached at the fastest speed, the time needed by the temperature rising stage and the temperature falling stage is shortened, and oscillation and overshoot of temperature waveforms of PCR in the circulation section are reduced.

Description

technical field [0001] The invention relates to the technical field of PCR temperature control, in particular to a control method, a control device and a PCR detector for a rapid constant temperature of a PCR detector. Background technique [0002] Nucleic acid amplification technology currently includes conventional digital polymerase chain reaction technology, real-time fluorescent digital polymerase chain reaction technology, and isothermal nucleic acid amplification technology. Molecular biotechnology is used to amplify specific DNA molecular fragments. It uses DNA molecules as templates and a pair of artificially synthesized specific oligonucleotide primers to rapidly amplify specific DNA molecular fragments through the enzymatic reaction of DNA polymerase. biologically very important. The basic process of PCR reaction is divided into three steps. The first step is DNA denaturation (94°C), the hydrogen bonds of the double-stranded DNA template are broken under the act...

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Problems solved by technology

However, it has the disadvantages of large overshoot, slow temperature change, and long constant temperature time.

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