Odor receptor derived peptide capable of effectively monitoring oriental migratory locust and biosensor thereof

A technology of odorant receptors and derived peptides, applied in hormone receptors, receptors/cell surface antigens/cell surface determinants, instruments, etc., can solve problems such as unpredictability and achieve the effect of small response

Pending Publication Date: 2022-07-01
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Homology modeling to predict the three-dimensional structure of the protein requires at least 25% similarity between the target sequence and the template, so homology modeling cannot be used to predict the structure of other insect odorant receptors

Method used

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  • Odor receptor derived peptide capable of effectively monitoring oriental migratory locust and biosensor thereof
  • Odor receptor derived peptide capable of effectively monitoring oriental migratory locust and biosensor thereof
  • Odor receptor derived peptide capable of effectively monitoring oriental migratory locust and biosensor thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Test materials and methods for making odorant receptor-derived peptide sensors

[0017] 1. Selection and structure prediction of odorant receptors

[0018] Aggregation pheromones are very important pheromones released by gregarious insects that cause aggregation behaviors in females and males of the species. East Asian migratory locusts gather and attract through odorant receptors that recognize aggregation pheromones. The gregarious East Asian locusts release aggregation pheromone, which is composed of 4-methoxystyrene (4-vinylanisole, 4VA). 4VA has a significant attracting effect on East Asian locusts in the field and laboratory. The binding sites for odorants are all on the transmembrane domain (TM) of the OR. Therefore, the odorant receptors speculate that the binding site is located on the transmembrane domain, and because the transmembrane domain of G protein-coupled receptors usually contains 21 hydrophobic amino acid sequences, its three-dimensional structure ...

Embodiment 2

[0052] Test results

[0053] 1. Odor receptor-derived peptide sensor detects 4VA standard

[0054] like Figure 4 Shown, the response graph of the odorant receptor-derived peptide sensor to 4VA gas, measuring the resistance change of the sensor in real time over multiple cycles.

[0055] In the figure a: the response of the odorant receptor-derived peptide 2 sensor to 4VA gas with a concentration of 10ppb to 100ppb;

[0056] b in the figure: the response of the odorant receptor-derived peptide 3 sensor to 4VA gas with a concentration of 1 ppb to 100 ppb;

[0057] In this embodiment, one measurement cycle includes gas in and gas out states. When the gas enters the state, the closed detection chamber is filled with 4VA gas. When the gas is discharged, flush the detection chamber with fresh air until there is no 4VA. The resistance of the sensor without the odorant receptor-derived peptide did not change under different species and concentrations of 4VA, suggesting that bare ...

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Abstract

The invention discloses an odor receptor derived peptide capable of effectively monitoring oriental migratory locust and a biosensor of the odor receptor derived peptide, and discloses application of the odor receptor derived peptide to preparation of equipment capable of effectively monitoring the occurrence condition of oriental migratory locust disasters, and the odor receptor derived peptide has an amino acid sequence as shown in SEQ ID NO: 2 or 3. The invention further discloses the biosensor, the biosensor comprises a substrate and an interdigital electrode, a single-walled carbon nanotube is firmly attached to the interdigital electrode, and the biosensor further comprises the sex pheromone receptor derived peptide connected with the single-walled carbon nanotube. According to the invention, specific monitoring can be carried out on oriental migratory locust, aggregation pheromones released by oriental migratory locust can be specifically identified, and response to host volatile matters is small. Under laboratory conditions, aggregation pheromones released by one oriental migratory locust can be sensitively monitored in a test box of 12000 cm < 3 >.

Description

technical field [0001] The invention relates to an odorant receptor-derived peptide and a biosensor which can effectively monitor the East Asian locust. Background technique [0002] The East Asian locust (Locusta migratoria manilensis) is one of the most important agricultural pests in grasslands and farmland. It has received a lot of attention from researchers because it can erupt and wreak havoc on agriculture. Individuals with low density are called scattered East Asian migratory locusts, and groups with high density are called gregarious types. Harmful to wheat, corn, rice, sugarcane, cotton and other 2 families and dozens of crops. L. migratoria can migrate over long distances, and the gregarious locusts are destructive to crops, pastures and other green vegetation. [0003] For many years, this pest has been mainly controlled by the application of various pesticides, but it has also caused environmental pollution. To control locust invasions, potential surveillanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/72G01N27/327
CPCC07K14/723G01N27/3275
Inventor 秦国轩王治陈瑞冰严善春马维超
Owner TIANJIN UNIV
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