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Preparation method of procyanidine-chitosan microgel

A technology of procyanidin and chitosan, which is applied in the field of preparation of procyanidin-chitosan microgels, can solve the problem of difficulty in accurately controlling rheological properties, large differences in emulsification properties of natural functional factors, particle size, surface charge and other problems. It is difficult to control the spatial microstructure and other problems, so as to achieve the effect of not easy to stratify or precipitate, easy to precipitate and precipitate, and to optimize the dispersion characteristics and emulsification characteristics.

Pending Publication Date: 2022-07-29
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to accurately control the rheological properties by using a single protein or macromolecular polysaccharide, so the emulsification properties of different fat-soluble natural functional factors are quite different, and its particle size, surface charge and spatial microstructure are also difficult to control

Method used

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  • Preparation method of procyanidine-chitosan microgel
  • Preparation method of procyanidine-chitosan microgel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, a kind of preparation method of procyanidin-chitosan microgel, carry out the following steps successively:

[0054] 1) Accurately weigh 400mg grape seed proanthocyanidins (referred to as proanthocyanidins), the purity is ≥98%, and its average degree of polymerization is 6.5;

[0055] The above procyanidins were added to 50ml of ultrapure water, stirred with a magnetic stirrer until the procyanidins were completely dissolved, and a 5ml syringe was used to filter the solution with a 0.45um inorganic membrane to obtain a procyanidin solution with a concentration of 8mg / ml;

[0056] 2), accurately weigh 400mg of chitosan, its average molecular weight is 20kD, and the degree of deacetylation is 90-95%;

[0057] The above-mentioned chitosan was added to 50 mL of 1% glacial acetic acid aqueous solution, and a magnetic stirrer was used to stir until the chitosan was completely dissolved to obtain a chitosan solution with a concentration of 8 mg / ml;

[0058] 3), q...

Embodiment 2

[0059] Embodiment 2, a kind of preparation method of procyanidin-chitosan microgel, carry out the following steps in sequence:

[0060] 1), accurately weigh 400mg grape seed proanthocyanidins, the purity is ≥98%, and its average degree of polymerization is 10.6;

[0061] The above procyanidins were added to 50ml of ultrapure water, stirred with a magnetic stirrer until the procyanidins were completely dissolved, and a 5ml syringe was used to filter the solution with a 0.45um inorganic membrane to obtain a procyanidin solution with a concentration of 8mg / ml;

[0062] 2), accurately weigh 400mg chitosan, its average molecular weight is 40kD, and the degree of deacetylation is 80-85%;

[0063] The above-mentioned chitosan was added to 50 mL of 1% glacial acetic acid aqueous solution, and a magnetic stirrer was used to stir until the chitosan was completely dissolved to obtain a chitosan solution with a concentration of 8 mg / ml;

[0064] 3), take 15ml of procyanidin obtained in s...

Embodiment 3

[0065] Embodiment 3, a kind of preparation method of procyanidin-chitosan microgel, carry out the following steps in sequence:

[0066] 1), accurately weigh 400mg grape seed proanthocyanidins, the purity is more than 98%, and its average degree of polymerization is 3.2;

[0067] The above procyanidins were added to 50ml of ultrapure water, stirred with a magnetic stirrer until the procyanidins were completely dissolved, and a 5ml syringe was used to filter the solution with a 0.45um inorganic membrane to obtain a procyanidin solution with a concentration of 8mg / ml;

[0068] 2), accurately weigh 400mg of chitosan, its average molecular weight is 10kD, and the degree of deacetylation is 90-95%;

[0069] The above-mentioned chitosan was added to 50 mL of 1% glacial acetic acid aqueous solution, and a magnetic stirrer was used to stir until the chitosan was completely dissolved to obtain a chitosan solution with a concentration of 8 mg / ml;

[0070] 3), take 5ml of procyanidin obt...

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Abstract

The invention discloses a preparation method of procyanidine-chitosan composite microgel, which comprises the following steps: dissolving procyanidine in ultrapure water, and filtering by using a 0.45 mu m filter membrane to obtain a procyanidine solution; dissolving chitosan in a glacial acetic acid aqueous solution to obtain a chitosan solution; and stirring the procyanidine solution obtained in the step 1) and the chitosan solution obtained in the step 2) at 500 + / -50 r / min for 4 + / -0.5 h according to the mass ratio of the procyanidine to the chitosan being 1: 1 to obtain the procyanidine-chitosan composite microgel. By adopting the method disclosed by the invention, the microgel with stable and appropriate rheological properties, particle size and microstructure can be prepared.

Description

technical field [0001] The invention relates to a preparation method of gel, in particular to a preparation method of procyanidin-chitosan microgel. Background technique [0002] Proanthocyanidins are natural active ingredients contained in many plants, and there are many reports that procyanidins have various physiological activities. Procyanidins are formed by the condensation of different amounts of epicatechin or catechin through C4-C6 or C4-C8. The simplest procyanidins are self-condensation of catechin or epicatechin or catechin. Dimers formed together with epicatechin can also form trimers, tetramers, and even decamers with each other. Proanthocyanidins have strong antioxidant activity, and the antioxidant capacity of oligomeric proanthocyanidins is 50 times that of vitamin E and 20 times that of vitamin C. There have been many reports that proanthocyanidins have various physiological activities, such as antioxidant, anti-tumor, anti-inflammatory, cardiovascular pro...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L29/275A23L2/52A23P10/30A23L29/10
CPCA23L33/105A23L29/275A23L2/52A23P10/30A23L29/10A23V2002/00A23V2200/228A23V2200/222A23V2200/30A23V2250/21166A23V2250/511
Inventor 葛建朱圣男万旺军徐爱春邓同乐刘军管峰
Owner CHINA JILIANG UNIV
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