Application of dbcAMP in preparation of primordial follicle in-vitro activator

A technology of primordial follicles and preparations, which is applied in the application field of dbcAMP in the preparation of primordial follicle activators in vitro, which can solve the problems of increasing patient pain, hidden dangers of drug safety and success rate, long operation time, etc., and achieve the effect of promoting activation

Pending Publication Date: 2022-08-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing IVA clinical strategies, the safety and success rate of related drugs are still a major hidden danger. At the same time, patients need to undergo multiple laparoscopic operations, which takes a long time and increases the pain of patients.

Method used

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  • Application of dbcAMP in preparation of primordial follicle in-vitro activator
  • Application of dbcAMP in preparation of primordial follicle in-vitro activator
  • Application of dbcAMP in preparation of primordial follicle in-vitro activator

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Experimental materials:

[0051] 2-day-old ICR female mice and 23-day-old ICR female mice.

[0052] 2. Experimental reagents:

[0053] Reagent A: Ovarian basal medium, the specific formula is shown in Table 1, and the preparation process must be performed aseptically.

[0054] Table 1 Ovarian basal medium formula

[0055] DMEM 0.5g F12 0.53g HEPES 0.357g NaHCO3 0.2438g penicillin 0.006g Streptomycin 0.005g Ultra-pure water Dilute to 100mL

[0056] Reagent B: 10 mM dbcAMP (solvent is ovarian basal medium)

[0057] Reagent C: Sterile PBS

[0058] Reagent D: Matrigel

[0059] 3. Experimental process

[0060] (1) Add 3 mL of Reagent A and 3 μL of Reagent B to the 6-well plate in turn, place a cell culture membrane in each well, and then place it in a cell incubator to preheat at 37°C;

[0061] (2) The ovaries of 2-day-old mice were separated in reagent C and then transferred to the culture membrane of step 1, and ...

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Abstract

The invention relates to the technical field of biology, in particular to application of dbcAMP in preparation of a primordial follicle in-vitro activator. The invention provides an application of a small molecule drug dbcAMP in preparation of a primordial follicle activated preparation. Experiments show that the dbcAMP can improve the phosphorylation level of key protein on a PI3K / mTOR signal pathway when the mouse ovary is cultured in vitro, so that the effect of the signal cascade pathway is amplified, and the activation of primordial follicles is further promoted. Meanwhile, in-vivo ovary in-situ injection of dbcAMP can stimulate activation of primordial follicles.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of dbcAMP in the preparation of primordial follicle activator in vitro. Background technique [0002] Assisted reproductive technology is the most effective means of assisted reproduction at this stage. A prerequisite for traditional assisted reproductive technology is mature and healthy oocytes, which requires the presence of growing follicles in the ovaries of female patients that can respond to gonadotropins. However, in patients with premature ovarian failure, polycystic ovary syndrome, and cancer after radiation / chemotherapy, there are not enough growing follicles in the ovaries that can respond to gonadotropins, making it difficult to obtain mature oocytes. [0003] To solve this problem, traditional assisted reproductive technology must be improved. In fact, these patients still have a certain number of primordial follicle resources in their ovaries, so wheth...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N5/075A61K31/7076A61P15/08
CPCC12N5/0609A61K31/7076A61P15/08C12N2501/727
Inventor夏国良王超郑文影张拓
OwnerCHINA AGRI UNIV