Stepped prepn of natural active protein and peptide

Abstract

The graded preparation method of natural active protein and peptide is characterized in that the protein-rich natural raw material is degreased, washed and homogenized, and 100 parts by weight of the homogenized raw material and 100-250 parts by weight of deionized water are added Into the biochemical reactor, adjust the pH to 5-8, raise the temperature to 70-90°C, keep warm and stir for 10-30 minutes, then quickly cool down to 30-50°C; add 0.02-0.5 parts by weight of protease, stir evenly, and carry out enzyme Hydrolysis reaction to obtain a mixture of proteins and peptides; remove particles through a 0.2 μm microporous membrane, and use polyethersulfone ultra-low protein adsorption membrane treated with OMEGA technology and tangential flow ultrafiltration technology to decompose the active protein after enzymatic hydrolysis Fractionation and concentration with peptides, control the operating temperature 20-30°C, pressure 0.1-0.25Mpa, pH=5-7, time 2-5 hours; select the upper and lower cut-off limits of the ultrafiltration membrane, and perform ultrafiltration in sequence to obtain different classification levels Molecular weight and structure of natural active proteins and peptides.

Description

1. Technical field [0001] The invention relates to a fractional preparation method of natural active proteins and peptides, which belongs to the field of biological product processing. 2. Background technology [0002] In recent years, the research on proteins and peptides has progressed rapidly, and many natural bioactive proteins and peptides have been isolated from animals, plants, bacteria, and fungi. Regardless of structure and function, natural bioactive proteins and peptides are the most complex compounds in nature. A large number of studies have confirmed that active proteins and peptides have the effects of promoting immunity, hormones, enzyme inhibitors, antibacterial, and antilipidemia. With the deepening of research, the physiological and pharmacological functions of more biologically active proteins and peptides have been gradually confirmed. [0003] Active proteins and peptides extracted from natural protein raw materials are a mixture of proteins and peptid...

AI Technical Summary

Problems solved by technology

Therefore, the fractional preparation of natural active proteins and peptides according to different application purposes is an important technology for the deep development and utilization of natural proteins and peptides. However, no reports on the preparation methods of natural active proteins and peptides have been reported so far.