Roguing and seed stock breeding method for transgenic insect-resisting cotton in seedling bed
An insect-resistant cotton and transgenic technology, which is applied in the field of agricultural science, can solve the problems of decreased insect resistance of cotton seeds, mixing of biology and machinery, etc., and achieves the effects of simple steps, strong operability, and simple and efficient operation.
Inactive Publication Date: 2005-12-28
江苏省农业科学院经济作物研究所
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[0011] The purpose of the present invention is to provide a solution to the technical problem that the insect resistance of cotton seeds has declined due to the mixing of biology and machinery in the current planting process of insect-resistant cotton, especially the loss of exogenous insect-resistant genes in the process of generation reproduction. A method for breeding transgenic insect-resistant cotton seedbeds to remove hybrids, thereby ensuring the purity of insect-resistant cotton seeds, maintaining and prolonging the lifespan of insect-resistant transgenic insect-resistant cotton
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[0033] The breeding method of the transgenic insect-resistant cotton seedbed of the present embodiment removes hybrids and improved varieties, taking the good propagation method of providing an annual output of about 800,000 kilograms of insect-resistant cotton seeds as an example, and its specific implementation steps are:
[0034] (1) Seed soaking—use aminoglycoside antibiotics (kanamycin, neomycin, paromomycin, etc.) to prepare a seed soaking aqueous solution with a concentration of 107 units / L. Add the seed soaking liquid to the propagated seeds, shake them well, and immerse them in the liquid. The soaking temperature is 25°C±2°C, and the soaking time is about 24 hours. Sow directly or soak and dry at another time.
[0035] (2) Seedling cultivation in nutrient pots - at the end of March or early April when the temperature is stable above 15°C, seedlings are sown in nutrient pots, and the seedbeds are covered with transparent plastic films (or covered with plastic sheds) to...
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The invention relates to a breeding method for insect-resistant cotton varieties, which belongs to the field of agricultural science and technology. The method is to soak seeds with chemical reagents, grow seedlings in nutrient pots, remove macular miscellaneous seedlings in the seedbed, transplant, select plants and test seeds, remove hybrids and self-breed the mixed plant groups, store the self-bred seeds in cold storage for future use, and produce original seeds according to a certain reproduction coefficient, etc. step. The entire good propagation procedure, through the seedbed removal of impurities in three growth cycles, achieves the purpose of ensuring the purity of insect-resistant cotton seeds, maintaining and prolonging the insect-resistant life of transgenic insect-resistant cotton, and solving the biological and mechanical problems in the planting process of insect-resistant cotton. Mixing, especially the loss of exogenous insect-resistant genes in the process of generation reproduction, which causes the technical problem of the decline of cotton seed insect resistance. The method adopted by the invention to maintain the purity of the insect-resistant cotton is simple, intuitive and practical, and the breeding program of the improved variety is highly operable, and is particularly suitable for popularization and application in the agricultural field.
Description
technical field [0001] The invention relates to a method for breeding improved varieties of insect-resistant cotton, in particular to a method for breeding improved varieties of insect-resistant cotton by soaking seeds with specific chemical reagents and removing impurities from seedbeds, and belongs to the field of agricultural science and technology. Background technique [0002] Bt cotton is one of the crops currently approved for commercial production in China. Transgenic insect-resistant cotton plays a positive role in many aspects such as curbing cotton insect damage, saving agricultural costs, reducing labor intensity, reducing environmental pollution caused by spraying pesticides, and promoting the improvement of farmland micro- and macro-environment. workers' attention [1，2，3，4] . [0003] Cotton is a frequently cross-pollinated crop, and natural biological hybridization and mechanical hybridization can easily cause variety degeneration. Due to the many productio...
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IPC IPC(8): A01H1/04
Inventor 陈旭升狄佳春许乃银刘剑光肖松华
Owner 江苏省农业科学院经济作物研究所
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