Roguing and seed stock breeding method for transgenic insect-resisting cotton in seedling bed

An insect-resistant cotton and transgenic technology, which is applied in the field of agricultural science, can solve the problems of decreased insect resistance of cotton seeds, mixing of biology and machinery, etc., and achieves the effects of simple steps, strong operability, and simple and efficient operation.

Inactive Publication Date: 2003-06-25
江苏省农业科学院经济作物研究所
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Problems solved by technology

[0011] The purpose of the present invention is to provide a solution to the technical problem that the insect resistance of cotton seeds has declined due to the mixing of biology and machinery in the current planting process of insect-resistant cotton, especially the loss of exogenous insect-resistant genes in the process of generation reproduction. A method for breeding transgenic insect-resistant cotton seedbeds to remove hybrids, thereby ensuring the purity of insect-resistant cotton seeds, maintaining and prolonging the lifespan of insect-resistant transgenic insect-resistant cotton

Method used

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  • Roguing and seed stock breeding method for transgenic insect-resisting cotton in seedling bed
  • Roguing and seed stock breeding method for transgenic insect-resisting cotton in seedling bed

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Embodiment

[0033] The breeding method of the transgenic insect-resistant cotton seedbed of the present embodiment removes hybrids and improved varieties, taking the good propagation method of providing an annual output of about 800,000 kilograms of insect-resistant cotton seeds as an example, and its specific implementation steps are:

[0034] (1) Seed soaking——prepared with aminoglycoside antibiotics (kanamycin, neomycin, paromomycin, etc.) for 10 7 Seed soaking water solution with unit / L concentration. Add the seed soaking liquid to the propagated seeds, shake them well, and immerse them in the liquid. The soaking temperature is 25°C±2°C, and the soaking time is about 24 hours. Sow directly or soak and dry at another time.

[0035] (2) Seedling cultivation in nutrient pots - at the end of March or early April when the temperature is stable above 15°C, seedlings are sown in nutrient pots, and the seedbeds are covered with transparent plastic films (or covered with plastic sheds) to mai...

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Abstract

The present invention relates to agricultural technology. The rogusing and seed stock breeding process includes soaking seed in chemical reagent, raising seedling in nutritive pot, eliminating seedlings with yellow spot, transplanting, selecting plant and seed, roguing and selfing, cold storing of selfing seed, producing original seed in certain propagation coefficient and other steps. Through roguing in the seedling bed for three breeding periods, the present invention can purify insect-resisting cotton seed, maintain and prolong insect-resisting performance life, prevent biological and mechanical mixing and especially prevent the degradation of insect-resisting performance caused by the loss of foreign insect-resisting gene.

Description

technical field [0001] The invention relates to a method for breeding improved varieties of insect-resistant cotton, in particular to a method for breeding improved varieties of insect-resistant cotton by soaking seeds with specific chemical reagents and removing impurities from seedbeds, and belongs to the field of agricultural science and technology. Background technique [0002] Bt cotton is one of the crops currently approved for commercial production in China. Transgenic insect-resistant cotton plays a positive role in many aspects such as curbing cotton insect damage, saving agricultural costs, reducing labor intensity, reducing environmental pollution caused by spraying pesticides, and promoting the improvement of farmland micro- and macro-environment. workers' attention. [0003] Cotton is a frequently cross-pollinated crop, and natural biological hybridization and mechanical hybridization can easily cause variety degeneration. Due to the many production steps of c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
Inventor 陈旭升狄佳春许乃银刘剑光肖松华
Owner 江苏省农业科学院经济作物研究所
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