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Method for separating biological macromolecule by using two-dimensional or multi-dimensional capillary electrophoresis

A technology of biological macromolecules and capillary electrophoresis, which is applied in the direction of material analysis, analysis materials, and measuring devices through electromagnetic means, can solve the problems of two-dimensional or multi-dimensional capillary electrophoresis that have not been reported, and achieve the effect of optimizing the separation effect

Inactive Publication Date: 2006-02-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

But so far, two-dimensional or multi-dimensional capillary electrophoresis with high column counts and the combination of different modes have not been reported.

Method used

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  • Method for separating biological macromolecule by using two-dimensional or multi-dimensional capillary electrophoresis
  • Method for separating biological macromolecule by using two-dimensional or multi-dimensional capillary electrophoresis
  • Method for separating biological macromolecule by using two-dimensional or multi-dimensional capillary electrophoresis

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Experimental conditions:

[0026] Capillary column (Hebei Yongnian Ruiyang Chromatography Device Co., Ltd., 50 μm I.D., 375 μm O.D.), carrier ampholyte Pharmalyte (pH3.0-10.0, BioChemika, Switzerland), biomacromolecule component is hemoglobin, product of Shanghai Institute of Biochemistry, others (Its reagent name of the present invention) reagent is analytically pure.

[0027] Capillary Electrophoresis for Unimicro Technology TriSep TM -2000GV type, including a Data Module tunable wavelength ultraviolet-visible detector, a CEC control module continuously adjustable high-voltage DC power supply; data collection and spectrogram processing use Echrom98 workstation and Echrom98 V2.0 software of Elite Company.

[0028] The special interface of the method for realizing two-dimensional or multi-dimensional capillary electrophoresis separation of biomacromolecules has an interface pool body 2, and an electrode socket 4, an inlet 5, and an outlet 6 are arranged on the interfac...

Embodiment 2

[0038] The difference from Example 1 is that the online removal of the buffer solution containing low molecular weight substances is the same as in Example 1. When implementing the second dimension capillary gel electrophoresis separation (voltage 8kV), on the first dimension capillary column No voltage is applied, and the pressure of 0.1Mpa is used to drive the peak; when the third-dimensional capillary column gel electrophoresis separation is carried out (voltage 8kV), no voltage is applied to the first and two-dimensional capillary columns, and the voltage is replaced by A pressure of 0.1 MPa, used to drive the peaks, yielded similar results.

[0039] Related comparative examples

[0040] The schematic diagram of the multi-mode and multi-dimensional analysis composed of gel chromatography / liquid chromatography / capillary electrophoresis designed by Jorgenson et al. uses a three-way valve interface and cannot be operated continuously. In addition, it requires high performance...

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Abstract

The present invention relates to a method for separating biological macromolecular component by using two-dimensional or multidimensional capillary electrophoresis and its interface. The biological macromolecular component undergone the process of one-dimensional capillary electrophoresis separation can be passed through the interface and fed into two-dimensional capillary electrophoretic separation operation to implement two-dimensional capillary electrophoresis, the small molecular substance can be diffused and freely permeated through the hollow fibre in the interface and can obtain balance in it, and when the described biological macromolecular component is flowed through the interface, the buffer solution containing low molecular substance can be on-line added / removed into the hollow fibre in the interface.

Description

technical field [0001] The invention relates to capillary electrophoresis technology, in particular to a two-dimensional or multi-dimensional capillary electrophoresis method for separating biomacromolecules. Background technique [0002] Capillary electrophoresis (CE) is a simple and efficient separation and analysis method. Compared with the plate electrophoresis method, it has many advantages such as rapidity, less sample consumption, and easy automation. It has been more and more widely used in the separation and analysis of biologically active substances such as proteins, antibodies, and clinical samples. [0003] Although the capillary electrophoresis method has a high column efficiency, it is generally a one-dimensional mode. Due to the limitation of the applied voltage, the length of the separation column used is restricted, which also limits the actual column efficiency and possible peak capacity. In addition, the too small inner diameter of the capillary column ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N33/561
Inventor 张玉奎杨春张凌怡张维冰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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