Capillary electrophoresis multi dimension device
A technology of capillary electrophoresis and capillary, which is applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of hollow fiber and capillary column dead angle, large loss of column efficiency, large dead volume, etc., and achieve simple production process, High column efficiency and low cost effect
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[0037] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.
[0038] Capillary column (Hebei Yongnian Optical Fiber Factory, 50 μm I.D., 375 μm O.D.), carrier ampholyte (pH 3.0-10.0, BioChemika, Switzerland), γ-methacryloxypropyltrimethoxysilane (γ-MAPS, 98 %), acrylamide (Acrylamide), N, N, N', N'-tetramethylethylenediamine (TEMED, 98%), ammonium persulfate (APS, +98%) (U.S., Bailingwei Company), Biomacromolecular components are cytochrome c (Cytochrome c), myosin (Myoglobin) and hemoglobin (Hemoglobin) (products of Shanghai Institute of Biochemistry), and other reagents are analytically pure. All buffer solutions and sample solutions must be filtered with a 0.45 μm microporous membrane before use, and degassed by ultrasonic before use.
[0039] The capillary electrophoresis instrument is the TriSepTM-2010GV model from Unimicro Technologies, including a Data Module adjustable wavelength UV-visible dete...
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