Capillary electrophoresis multi dimension device

A technology of capillary electrophoresis and capillary, which is applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of hollow fiber and capillary column dead angle, large loss of column efficiency, large dead volume, etc., and achieve simple production process, High column efficiency and low cost effect

Inactive Publication Date: 2006-01-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Due to the mismatch between the inner diameter of the hollow fiber and the capillary column and the dead angle caused by the inability to connect the hollow fiber and the capillary column tightly, this type of interface also has problems such as large dead volume and large loss of column efficiency during column switching.
[0014] All the above-mentioned interfaces have various defects, which ultimately limit their application in two-dimensional or multi-dimensional capillary electrophoresis systems.

Method used

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Embodiment Construction

[0037] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0038] Capillary column (Hebei Yongnian Optical Fiber Factory, 50 μm I.D., 375 μm O.D.), carrier ampholyte (pH 3.0-10.0, BioChemika, Switzerland), γ-methacryloxypropyltrimethoxysilane (γ-MAPS, 98 %), acrylamide (Acrylamide), N, N, N', N'-tetramethylethylenediamine (TEMED, 98%), ammonium persulfate (APS, +98%) (U.S., Bailingwei Company), Biomacromolecular components are cytochrome c (Cytochrome c), myosin (Myoglobin) and hemoglobin (Hemoglobin) (products of Shanghai Institute of Biochemistry), and other reagents are analytically pure. All buffer solutions and sample solutions must be filtered with a 0.45 μm microporous membrane before use, and degassed by ultrasonic before use.

[0039] The capillary electrophoresis instrument is the TriSepTM-2010GV model from Unimicro Technologies, including a Data Module adjustable wavelength UV-visible dete...

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Abstract

The present invention relates to capillary electrophoresis multidimensional equipment. Said equipment includes the following components: an electrophoretic bath body, a capillary tube and a high-voltage DC power supply. Said invention also provides the concrete structure of the above-mentioned every component and connection mode of all the above-mentioned components.

Description

technical field [0001] The invention relates to a capillary electrophoresis multidimensional device. Background technique [0002] Capillary electrophoresis (CE) is a simple and efficient separation and analysis method. Compared with the plate electrophoresis method, it has many advantages such as high efficiency, rapidity, less sample consumption, and easy automation. It has been more and more widely used in the separation and analysis of biologically active substances such as proteins, antibodies, and clinical samples. [0003] Although the capillary electrophoresis method has high column efficiency, the one-dimensional separation mode, due to the limitation of its applied voltage, restricts the length of the separation column used, thus limiting the actual column efficiency and possible peak capacity. In addition, the too small inner diameter of the capillary column makes the optical path of its spectral detection too small, which has a great impact on the detection sens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/453G01N27/447
Inventor 张玉奎刘和春朱贵杰张丽华张维冰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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