Growth hormone conjunctive protein activity determination kit, preparation and determination method

Abstract

This invention discloses a determination kit box combining with protein testing the growing hormone activity in and animals and its preparation and test method characterizing that the box is composed of a solution prepared by a labeled growing hormone, unlabeled growing hormone, glucose enveloped carbon, general buffer solution and analytical solution and a specification. The method is that apart from setting 4-8 NSB tubes, two correspondent NSB tubes are specially designed for every sample to make the test more accurate. The glucose enveloped carbon preparation and quatily are necessary conditions for the accurate test.

Description

1. Technical field

[0001] The invention relates to a medical index assay kit for assaying growth hormone binding protein activity in human and animal serum and its preparation and assay methods. 2. Background technology

[0002] Growth hormone binding protein (GHBP) in human and animal serum plays an important role in the distribution, metabolism and physiological activities of growth hormone. GHBP is a single-chain protein composed of 238-246 amino acids, with a molecular weight of about 61kDa, an acidic protein, and an isoelectric point of about 5.0. The main physiological function of GHBP is to combine with the growth hormone produced by the pituitary gland to transport the growth hormone to the tissue cells of the whole body in the blood, which can effectively store the growth hormone and prolong the activity of the growth hormone. GHBP can also combine with the growth hormone and the growth hormone receptor The increase and decrease of growth hormone regulate the physi...