Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining gastrodine content

A determination method, the technology of gastrodin, which is applied in the field of determination of gastrodin content, can solve the problems of less research on the extraction process, and achieve the effect of improving the extraction rate and the measured value

Inactive Publication Date: 2004-07-28
贵州宏宇药业有限公司 +1
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the extraction process of Gastrodia elata test solution from Gastrodia elata medicinal materials.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1. Determination according to high performance liquid chromatography ("Chinese Pharmacopoeia" 2000 edition appendix VID).

[0012] Chromatographic conditions and system suitability test: the same as the "Chinese Pharmacopoeia" (2000 edition). Use octadecyl bonded silica gel as filler; methanol-phosphate solution (0.1mol / L potassium dihydrogen phosphate solution and 0.1mol / L disodium hydrogen phosphate solution are mixed in equal amounts)-water (4:2:94) It is the mobile phase; the detection wavelength is 270nm; the flow rate: 1.0ml / min; the column temperature: 25°C; the number of theoretical plates should not be less than 1500 based on the gastrodin peak.

[0013] Preparation of the test solution: Accurately weigh 0.25g of Gastrodia elata powder dried at 80°C to constant weight, put it in a 10ml measuring bottle, weigh it, add 7.5ml of methanol with a concentration of 40%, ultrasonically treat it for 45 minutes, and let it stand After 6 hours, ultrasonic treatm...

Embodiment 2

[0017] Example 2. Preparation of the test solution: Accurately weigh 0.25 g of Gastrodia elata powder dried at 80°C to constant weight, put it in a 10ml measuring bottle, weigh it, add 5ml of methanol with a concentration of 60%, ultrasonically treat it for 15 minutes, and let it stand for 6 Hours, ultrasonic treatment for 15 minutes, weigh again, make up the lost weight with 60% methanol, shake well, let stand, take the supernatant and centrifuge to get the test solution. Others are the same as embodiment 1.

[0018] The measurement results show that, for the same Gastrodia elata sample, the gastrodin content measured by the method of this example is significantly higher than that of the method of "Chinese Pharmacopoeia" (2000 edition), which can be increased by nearly 16 times.

Embodiment 3

[0019] Example 3. Preparation of the test solution: Accurately weigh 0.25 g of Gastrodia elata powder dried at 80°C to constant weight, put it in a 10ml measuring bottle, weigh it, add 10ml of methanol with a concentration of 20%, ultrasonically treat it for 15 minutes, and let it stand for 6 Hours, then sonicate for 15 minutes, weigh again, make up the lost weight with 20% methanol, shake well, let stand, take the supernatant and centrifuge to get the test solution. Others are the same as embodiment 1.

[0020] The measurement results show that, for the same Gastrodia elata sample, the gastrodin content measured by this method is significantly higher than that of the method of "Chinese Pharmacopoeia" (2000 edition), which can be increased by nearly 17 times.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present inventino discloses a method for determining gastrodine content. It adopts the following method to prepare test sample solution: precisely weighing gastrodia root powder dried to constant weight, placing it in graduated flask, calibrating weight, precisely adding methyl alcohol whose concentration is 20%-90% and added quantity is 20-40 times gastrodia root powder weight, making ultrasonic treatment for 15-120 min, standing still for 0-24 hr., secondary ultrasonic treatment for 0-120 min, more calibrating weight, using methyl alcohol whose concentration is 20%-90% of supplement reduced weight, uniformly shaking them, standing still, taking supernatant fluid and centrifuging so as to obtain the invented test sample solution.

Description

technical field [0001] The invention relates to a method for determining the composition of a medicine, in particular to a method for determining the content of gastrodin. Background technique [0002] Gastrodia elata is a well-known commonly used traditional Chinese medicine. There are many Chinese patent medicines made from Gastrodia elata. The main component of Gastrodia elata is gastrodin, so there are many reports on the determination of gastrodin in Gastrodia elata. The methods for determining the content of gastrodin in Gastrodia elata mainly include reversed-phase high-performance liquid chromatography (RP-HPLC), dual-beam thin-layer chromatography scanning, capillary electrophoresis, and ultraviolet spectrophotometry, among which HPLC is the most widely used. "Chinese Pharmacopoeia" (2000 edition) stipulates that the HPLC method is used to determine the content of gastrodin under the content determination item of Gastrodia elata, which is used as the main index for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06G01N30/40G01N30/74
Inventor 梁光义阮婧华徐必学柴立李霞严崇文
Owner 贵州宏宇药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com