Seed separating and large scale culture method of toxic dinoflagellate

A large-scale culture and large-scale technology, applied in the fields of botanical equipment and methods, algae products, applications, etc., can solve the problems of large molecular weight of toxins, difficult to synthesize, etc. Effect

Inactive Publication Date: 2004-09-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these toxins tend to be large in molecular weight and difficult to synthesize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Collect 500 g of macroalgae in tropical coral reef waters, add 1000 ml of seawater, wash out the paraphytes, and collect the supernatant to obtain a water sample containing cells of Amphidinium klebsii algae. The surface water temperature at the sampling site was 24°C, the salinity was 35‰, and the pH was 8.048. For the isolated algal cells, use natural seawater, f / 2, silicon-free K / 2, and K culture medium to establish algal strains under the environmental conditions of 24±2°C, 5000-11000Lux natural light, and a light-dark cycle of 12 / 12h. The pure line of Amphidinium klebsii was obtained after repeated separation, domestication and cultivation in the laboratory, separation and purification.

Embodiment 2

[0021] Example 2: Collect 1000 g of macroalgae in tropical coral reef waters, add 2000 ml of seawater, wash out the epiphytes, collect the supernatant, and obtain a water sample containing Amphidinium klebsii algal cells. The surface water temperature at the sampling site is 25°C, the salinity is 35.3‰, and the pH is 8.050. For the isolated algal cells, use natural seawater, f / 2, silicon-free K / 2, and K culture medium to establish algal strains under the environmental conditions of 25±3°C, 5000-11000Lux natural light, and a light-dark cycle of 12 / 12h. The pure line of Amphidinium klebsii was obtained after repeated separation, domestication and cultivation in the laboratory, separation and purification.

Embodiment 3

[0022] Example 3: Collect 2000 g of macroalgae in tropical coral reef waters, add 3000 ml of seawater, wash out the paraphytes, and collect the supernatant to obtain a water sample containing cells of Amphidinium klebsii algae. The surface water temperature at the sampling site was 26°C, the salinity was 36‰, and the pH was 8.050. For the isolated algal cells, use natural seawater, f / 2, silicon-free K / 2, and K culture medium to establish algal strains under the environmental conditions of 27±2°C, 5000-11000Lux natural light, and a light-dark cycle of 12 / 12h. A pure line of Amphidinium klebsii was obtained after repeated separation, domestication and cultivation in the laboratory, separation and purification.

[0023] The use of the pure line of Amphidinium klebsii provided by the present invention can be used as a large-scale production to obtain sufficient raw materials to extract secondary metabolites - dinoflagellates.

[0024] Using the method of the invention to isolate ...

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PUM

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Abstract

The present invention is the seed separating way, culture method and application of Amphi dinium klebsii as one kind of toxic dinoflagellate. Research shows that there are 60-78 kinds of toxic marine algae and the toxic marine algae have wide application foreground in the research of cancer mechanism and the development of various medicines for resisting cancers, relieving pain, anaesthesia, resisting virus, resisting bacteria, etc. The toxic dinoflagellate under natural environment has very low cell density and this limits its application. For sake of obtaining sufficient material of toxic dinoflagellate, the present invention provides the method of separating pure Amphi dinium klebsii seed from natural water and proliferating in great amount for large scale production.

Description

technical field [0001] The invention relates to an isolation approach, a culture method and an application of the algae pure line of Amphidinium klebsii. Specifically, it is a method for pure species separation and large-scale production of toxic dinoflagellates. Background technique [0002] Dinotoxin is one of the fields with the fastest progress in the research and development of marine bioactive substances. There are about 60-78 species of algae that can produce toxins in the ocean, among which toxic dinoflagellates are the most, accounting for 73-75%. There are many types of dinoflagellates, the common ones are paralytic shellfish poisoning (PSP), diarrhea shellfish poisoning (DSP), amnesia shellfish poisoning (ASP), neuropathic shellfish poisoning (NSP) and Ciguatoxin (Ciguatoxin, Maitotoxin, Ostreotoxin) and other categories. Judging from the current research progress, dinoflagellates have very broad application prospects in the study of cancer mechanisms and the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H13/00
Inventor 龙丽娟吴军李庆欣张偲
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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