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Compositions and methods for inhibiting pathogenic growth

A technology of composition and food quantity, applied in botany equipment and methods, plant growth regulators, biocides, etc., can solve problems such as detection difficulties

Inactive Publication Date: 2014-07-09
CHR HANSEN AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, pathogenic organisms can cause disease in very low numbers, making detection very difficult

Method used

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  • Compositions and methods for inhibiting pathogenic growth
  • Compositions and methods for inhibiting pathogenic growth
  • Compositions and methods for inhibiting pathogenic growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 and 2

[0028] Several in vitro assays are performed to demonstrate that specific bacteria are capable of effectively competing or interfering with the growth of eg E. coli O157:H7 and other pathogenic bacteria.

Embodiment 1

[0030] Lyophilized cultures of lactic acid-producing and lactate-utilizing microorganisms were selected for their ability to inhibit the growth of pathogens such as E. coli O157:H7, Streptococcus aureus, and Salmonella. Combinations of lactate-producing and lactate-utilizing microorganisms were further selected for their greatest ability to inhibit the growth of multiple pathogens.

[0031] In order to identify those microorganisms that could be utilized in the methods and formulations of the present invention, in vitro experiments were performed to identify individual strains with significant effects. Seven propionic acid strains and six Lactobacillus strains were screened for their ability to produce bacteriocins capable of producing zones of inhibition on agar plates growing E. coli O157:H7. The test results are shown in the table below:

[0032] Table 1 Inhibitory activity of propionic acid bacteria strains grown on selective media

[0033]

[0034] Table 2 Inhibitory...

Embodiment 2

[0038] Selected Lactobacillus acidophilus and Propionibacterium freunderi were compared with E. coli for in vitro growth in a rich semi-anaerobic medium at 38°C to determine whether they could more effectively compete with E. coli under in vivo growth conditions strains. Strains LA51 and LA45 were found to grow faster than E. coli.

[0039] Table 3 Comparison of growth (absorbance) of selected bacterial strains and E. coli O157:H7 in rich semi-anaerobic medium at 38°C

[0040] minute Escherichia coli O157:H7 LA45 LA51 PF24 0 0.2 0.2 0.2 0.2 50 0.3 0.38 0.55 0.3 90 0.45 0.65 0.84 0.35 120 0.60 0.85 1.0 0.36 200 0.80 1.2 1.28 0.38 230 0.85 1.25 1.28 0.39 365 0.90 1.25 1.28 0.50 440 0.90 1.25 1.28 0.58

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Abstract

The invention includes methods and compositions for treating an animal to inhibit the incidence and growth of E. coli O157:H7 and other pathogenic bacteria. The treatment method comprises administering a therapeutically effective amount of Lactobacillus acidophilus or one or a combination of a number of other probiotic bacteria to an animal. An alternative treatment method comprises administering a therapeutically effective amount of a lactic acid producing bacterium such as Lactobacillus acidophilus in combination with a lactate utilizing bacterium such as Propionibacterium freudenreichii.

Description

technical field [0001] The present invention relates to compositions and methods for inhibiting the growth of pathogens. In particular, the present application relates to compositions and methods for inhibiting the growth of pathogens by using lactic acid producing microorganisms alone and in combination with lactate utilizing microorganisms. Background technique [0002] Ingestion of pathogens, especially bacterial pathogens, is a ubiquitous problem for most animals, in addition to including viruses as well as other pathogenic microorganisms. The pathogen is known to cause illness in animals, with a wide range of deleterious effects including: weight loss, diarrhea, abdominal cramps, and kidney failure. In immunosuppressed or malnourished animals, even diarrhea alone can be fatal. Pathogens can spread among animals in places with poor sanitation, and sometimes even the utmost care cannot prevent infectious diseases. The most common solution to the above problems is to pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00C12N1/20A23C9/12
Inventor 布赖恩·加纳道格拉斯·威尔
Owner CHR HANSEN AS