Protein marked by Tc-99 capable of specifically combined with GnRH-receptor expressed cancer cell

A cancer cell, -99m technology, applied to animal/human proteins, preparations for in vivo experiments, hybrid peptides, etc., can solve the problems of radionuclide inability, long physical half-life, unsuitable for scintigraphy quality requirements, etc.

Inactive Publication Date: 2006-05-10
BEIJING NORTHLAND BIOTECH
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

The advantages of other radionuclides used in the prior art are not as good as Tc-99m, because these radionuclides have longer physical half-lives, resulting in more radiation absorbed by the patient (eg Indium-111)
Or, the radiation energy of some other radionuclides is significantly lower (such as iodine-125) or significantly higher than (such as iodine-131) Tc-99m, so it is not suitable for the quality requirements of scintigraphic imaging. Suitable radionuclides cannot be produced with on-site generators

Method used

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  • Protein marked by Tc-99 capable of specifically combined with GnRH-receptor expressed cancer cell
  • Protein marked by Tc-99 capable of specifically combined with GnRH-receptor expressed cancer cell
  • Protein marked by Tc-99 capable of specifically combined with GnRH-receptor expressed cancer cell

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example

[0040] The recombinant expression plasmid containing GnRH protein gene is cloned by genetic engineering means and methods. After the plasmid was transformed into engineering bacteria, a high-purity GnRH protein sample was obtained through expression and purification, and then Tc-99m radiolabeled on GnRH in vitro, and the labeled protein was sterilized and injected intravenously into the tumor-bearing tumor. in mice. Scintigraphy was performed 2-4 hours after sample injection to visualize the location of the tumor mass.

[0041] The result is as figure 1 ,

[0042] Instance: 2

[0043] The recombinant expression plasmid containing the GnRH-PE40 fusion protein gene is cloned by genetic engineering means and methods. After the plasmid was transformed into engineering bacteria, a high-purity GnRH-PE40 fusion protein sample was obtained through expression and purification, and then the GnRH-PE40 fusion protein was radiolabeled with Tc-99m in vitro, and the labeled protein was s...

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Abstract

The invention relates to a protein in vivo detection agent for detecting the surface receptor types and cancer positions of the oncocyte in human body, which comprises specifically recognizing and expressing the paraprotein use technetium-99 label of the GnRH receptor's oncocyte, thus producing twinkling images in human body. The paraprotein is the guided fusion protein comprising natural GnRH, GnRH mutant, the PE toxin derivatives.

Description

1. Technical field [0001] Cancer is still a serious threat to human health, with high morbidity and mortality. There are three main methods of traditional treatment for cancer: chemotherapy, radiotherapy and surgery. Surgical therapy is limited to the early stage. Although chemotherapy and radiotherapy are effective, their serious side effects limit the use of large doses and long-term applications of this method, which greatly affects their therapeutic effect. [0002] Therefore, at this stage, people are committed to researching a new method of treating tumors with low toxicity and high efficiency, which is to eliminate the shortcomings of radiotherapy and chemotherapy methods that kill tumor cells while also killing a large number of normal cells, specifically killing tumor cells, and killing tumor cells. Low or no toxicity to normal cells. This method is the application of targeting molecules, including ligands of receptors overexpressed on the surface of tumor cells and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/00C07K19/00C12N15/63
CPCC07K14/61A61K51/088C07K7/23
Inventor 金美玉马素永聂李亚许日山
Owner BEIJING NORTHLAND BIOTECH
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