Variable area gene for single-clone antibody of anti-tumour necrotized factor with high neutralizing activity and preparation thereof

A monoclonal antibody and necrosis factor technology, applied in the field of biomedicine, can solve problems that easily cause immune reactions in the human body

Inactive Publication Date: 2006-05-31
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when these murine mAbs are used in humans, due to their immunogenicity, they are prone to induce immune responses in humans, which can cause clearance of antibodies and immune complex-mediated hypersensitivity reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Variable area gene for single-clone antibody of anti-tumour necrotized factor with high neutralizing activity and preparation thereof
  • Variable area gene for single-clone antibody of anti-tumour necrotized factor with high neutralizing activity and preparation thereof
  • Variable area gene for single-clone antibody of anti-tumour necrotized factor with high neutralizing activity and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The present invention will be further described in detail below in conjunction with the drawings.

[0036]The complete nucleotide sequence of the variable region gene, the nucleotide sequence of its functional fragment (such as CDR, etc.) and the amino acid sequence of the antibody variable region are the basis for the construction of chimeric antibodies and humanized antibodies. To this end, the applicant immunized BALB / c mice with recombinant human TNF and prepared a group of mouse anti-human TNF monoclonal antibodies. And use monoclonal antibodies to block TNF-induced apoptosis of L929 cells, monoclonal antibodies to block TNF-induced ICAM-1 expression in ECV304 cells, and monoclonal antibodies to block TNF-induced NF-κB nuclear translocation. D2 hybridoma cell line with high neutralizing activity. This strain of hybridoma cells has the ability to stably secrete antibodies.

[0037] 1. Technical scheme and route

[0038] 1.1 Preparation of mouse anti-human TNF high neutr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention presents a alterable gene preparation method of high neutralizations active high affinity anti-TNF-mAb(D6 mAb) mutable gene. It prepares a group little mouse anti-human single-clone antibody by regrouping human TNF immune BALB / c little mouse. From the interdiction experiment outside body, it can filtrate anti-human TNF single-clone antibody with high neutralization. From cloning this single-clone antibody heavy chains alterable gene, it gets its alterable gene and amino acid sequcence, and affirms the unique of its gene and albumen list. This invention select high neutralization D2 crossbreed cell line to clone heavy chains alterable gene successfully, and gets its special nucleotide list after testing. Coding the alterable gene list has huge potential value in forming human vector and gomphosis vector which can cure inflammation and self-immunity disease etc.

Description

Technical field [0001] The present invention belongs to the field of biomedical technology, and relates to a monoclonal antibody gene, in particular to a method for preparing a variable region gene of a high-neutralizing activity anti-tumor necrosis factor monoclonal antibody (D2 mAb). [0002] This application is the applicant's patent application on November 13, 2003, and its previous application number is 200310105919.9 with the same name as the divisional application of this application. Background technique [0003] Tumor Necrosis Factor (TNF) is a homotrimeric soluble protein composed of 3 subunits with a molecular weight of 17kDa, mainly produced by monocytes and macrophages. There is also a membrane-bound form of TNF with a molecular weight of 26 kDa in the body. TNF increases the adhesion of neutrophils and lymphocytes and stimulates the secretion of macrophages, neutrophils and vascular endothelial cells by inducing the expression of adhesion molecules such as intercell...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/13C07K16/18C12N5/18
Inventor 金伯泉刘雪松杨琨朱参胜
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap