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Mutagenesis method for breeding poplar

A technology for mutation breeding and new methods, which can be applied in horticultural methods, botanical equipment and methods, plant cells, etc., and can solve problems such as difficulty in realizing

Inactive Publication Date: 2007-05-09
上海光兆植物速生技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as poplar is concerned, it is difficult to achieve a certain amount if its seeds, branches, and single buds are used as treatment materials.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] A new poplar mutation breeding method, comprising selection of mutagenesis objects, selection of radiation timing and radiation treatment, wherein the mutagenesis objects are poplar callus embryogenic cells, and the radiation timing is poplar embryogenic cells Period of mass occurrence.

[0016] Obtain the step that poplar embryogenic cell takes place in large quantities:

[0017] The first step: select tissue culture age 40 days, European and American poplar (black poplar group) tissue culture seedling of seedling height 4~5cm, its stem section is cut into 1mm as explant;

[0018] Step 2: inoculate the stem segment on the dedifferentiation medium MS+2, 4D 0.2mg / L+NAA 0.1mg / L+sucrose 30g / L+agar 4g / L and dedifferentiate for 120 hours;

[0019] The third step: the callus obtained in the second step is transferred to the redifferentiation medium MS+6-BA0.2mg / L+KT 0.2mg / L+IAA 0.05+sucrose 30g / L+agar 4g / L and then differentiated for 72 hours , which is the period when the ...

Embodiment 2

[0022] A new poplar mutation breeding method, comprising selection of mutagenesis objects, selection of radiation timing and radiation treatment, wherein the mutagenesis objects are poplar callus embryogenic cells, and the radiation timing is poplar embryogenic cells Period of mass occurrence.

[0023] Obtain the step that poplar embryogenic cell takes place in large quantities:

[0024] The first step: select the stem section of poplar group cultured seedlings and cut them into 1-2 mm as explants;

[0025] The second step: inoculate the stem segment on the dedifferentiation medium MS+2,4-D 0-1.0mg / ml+KT0-0.5mg / ml+NAA 0-1.0mg / ml+ZT 0-0.5mg / ml Dedifferentiation for 72 to 120 hours;

[0026] The third step: the callus obtained in the second step is transferred to the redifferentiation medium MS+6-BA0-1.0mg / ml+KT 0-0.5mg / ml+IAA 0-1.0mg / ml+ZT 0-0.5mg / ml and then differentiated for 72 to 96 hours, reaching the time period when a large number of poplar embryogenic cells occurred...

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Abstract

A mutation breeding method for culturing a lot of variants of poplar tree includes such steps as choosing the embryonic cells of callus of poplar, choosing the time when said embryonic ells are greatly generated, and gamma ray irradiation by 20-50 Gy.

Description

technical field [0001] The new poplar mutation breeding method of the invention relates to a radiation-induced mutation method for poplar embryogenic cells, especially a new method for obtaining a large number of mutant plants through gamma-ray-induced mutation. Background technique [0002] The research and development of tree breeding shows that the traditional method has disadvantages such as long life span and slow breeding progress. The application of high technology combined with conventional breeding methods is the main direction of current tree breeding. [0003] Using physical and chemical factors to induce mutagenesis, select and breed excellent mutants, and cultivate them into varieties, which are widely used in variety improvement. The most important of these is to irradiate seeds or other reproductive organs with X-rays and γ-rays, but the mutation frequency after irradiation is extremely low (10 -8 -10 -5 ), so the number of processed individuals (seeds, buds...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H3/00A01H4/00C12N5/04
Inventor 苏敏宋学孟董举文张艳庞瑞华许雄山章振华王凌健张承妹
Owner 上海光兆植物速生技术有限公司
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