Electrochemical test strip for reducing the effect of direct and mediated interference current
a technology of direct and mediated interference current and electrochemical test strips, which is applied in the field of electrochemical strips and systems, can solve the problems of unsatisfactory oxidation current, and inability to achieve the effect of reducing the oxidation curren
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
[0095] Test strips 800 were prepared as illustrated in FIGS. 1 to 3a. Test strips 800 were tested in blood which were exposed to varying levels of sterilizing radiation. To test strips 800, they were electrically connected to a potentiostat which has the means to apply a constant potential of +0.4 volts between first working electrode 808 and reference electrode 810; and second working electrode 806 and the reference electrode 810. A sample of blood is applied to sample inlet 52 allowing the blood to wick into the sample receiving chamber and to wet first working electrode 808, reference electrode 810, and second working electrode 806. Active layer 820 becomes hydrated with blood and then generates ferrocyanide which may be proportional to the amount of glucose and / or interferent concentration present in the sample. In contrast, inactive layer 818 becomes hydrated with blood and does not generate additional ferrocyanide that was not present within inactive layer 818 before hydration...
example 2
[0096] Two batches of test strips were prepared to show that the use of inactive reagent layer 818 and active reagent layer 820 improved the overall precision for test strips sterilized by gamma radiation. Both batches of test strips were tested in a similar manner as described in Example 1. The first test strip batch is test strip 800 and is referred to as Batch 1. The second test strip batch, which is referred to as Batch 2, is also similar to test strip 800, but does not include inactive reagent layer 818 and also has a modified active reagent layer which covers both first working electrode 808, second working electrode 806, and reference electrode 810. When testing Batch 1, the difference in current from first working electrode 808 and second working electrode 806 was used to calculate a corrected signal current which was then converted to a glucose concentration. When testing Batch 2, the current from second working electrode 806 and first working electrode 808 were summed toge...
example
[0099] Another batch of test strips, which is referred to as Batch 3, was prepared in a manner similar to test strip 800 except that second working electrode 806 was not coated with either active reagent layer 820 or inactive reagent layer 818. In this example, Batches 1 to 3 were tested to evaluate the overall accuracy in the presence of interfering compounds such as uric acid and gentisic acid.
[0100] Batch 1, Batch 2, and Batch 3 test strips were tested in blood at three concentrations of gentisic acid which were 0, 25, and 50 mg / dL. For each gentisic acid concentration, two glucose concentrations were tested which were 70 and 240 mg / dL. FIGS. 16 and 17 show that Batch 1 and Batch 3 test strips had an insignificant change (2 test strips had a significant change (>10 mg / dL or 10%) in bias when testing them at a 25 and a 50 mg / dL gentisic acid concentration. This shows that the use of second working electrode 806 not coated with enzyme allows for an effective correction of the gluc...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More