Unlock instant, AI-driven research and patent intelligence for your innovation.

Genetic alterations associated with cancer

a gene and cancer technology, applied in the field of gene alterations associated with cancer, can solve the problems of limited homozygous (polymorphism-based techniques) and achieve the effect of reducing the number of homozygous

Inactive Publication Date: 2010-08-12
RGT UNIV OF CALIFORNIA +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Enables the early detection and prognosis of cancers by identifying specific genetic alterations in the 3q26.3 region, providing valuable diagnostic markers and potentially improving patient therapy and survival by targeting amplified genes like PIK3CA and AKT2.

Problems solved by technology

Moreover, because CGH does not rely on naturally occurring polymorphisms, all regions are informative, whereas polymorphism-based techniques are limited by homozygous (uninformative) alleles among a fraction of tumors studied at every locus.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetic alterations associated with cancer
  • Genetic alterations associated with cancer
  • Genetic alterations associated with cancer

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0095]This example describes the identification of genes in a 2 MB region at 3q26.3 region. Increased copy number of this region is correlated with ovarian cancer.

[0096]CGH studies have identified DNA copy number abnormalities at 3q25-26 associated with ovarian tumors (Iwabuchi et al., Cancer Research 55:6172-8180 (1995)). To physically map the 3q26 region, a number of yeast artificial chromosome (YAC), P1, and cosmid clones that were known to genetically map to the region were physically mapped to the 3q region. Several yeast artificial chromosome (YAC) and P1 clones known to genetically map to this region were physically mapped using FISH and fractional length analysis. Clone positions were reconfirmed by PCR using STSs specific to the region. Clones were then picked according to their physical map position and hybridized onto interphase cells of ovarian cancer, breast cancer, and melanoma cell lines, as well as nuclei of paraffin-embedded ovarian tumors. One of the P1 clones and ...

example 2

[0111]This example shows that PIK3CA expression is increased in ovarian cancers as a result of the PIK3CA copy number increase.

[0112]As noted above. PIK3CA is an attractive as a candidate oncogene in ovarian cancer because of the broad range of cellular functions that are modulated by increased PI3-kinase activity (Cantley et al. J. Am. Soc. Nephrol. 5:1872-1881 (1995), Fry Biochi. Biophys. Ada 1226:237-268 (1994)). These include increased including cell proliferation, accelarated glucose transport and catabolism (Frevert et al. Mol Cell Biol 117:190-198 (1997),Tsakiridis et al., Endocrinology 136:4315-4322 (1995), altered cell adhesion (Chen et al. J. Biol Chem. 269:31229-31233 (1994), Kinashi et al. Blood 86:2086-2090 (1995)) and altered vesicle transport (Joly et al. J. Biol. Chem. 270:13225-13230 (1995) and Dudek et al. Science 275:661-665 (1997)).

[0113]Increased PI-kinase activity also is implicated in abrogating apoptosis. For example, neuronal survival is increased after acti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Tmaaaaaaaaaa
temperatureaaaaaaaaaa
pHaaaaaaaaaa
Login to View More

Abstract

The present invention provides new probes for the detection of chromosomal alterations associated with cancer, particularly ovarian cancer. The probes bind selectively with target nucleic acid sequences at 3q26.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of copending application Ser. No. 08 / 905,508 filed Aug. 4, 1997, which is a continuation-in-part of application Ser. No. 08 / 837,046 filed Apr. 5, 1997, now U.S. Pat. No. 6,110,673, which is a continuation of application Ser. No. 08 / 783,729 filed Jan. 16, 1997, now U.S. Pat. No. 6,277,563, all of which are incorporated herein by reference.BACKGROUND OF THE INVENTION[0002]Molecular genetic mechanisms responsible for the development and progression of many cancers remain largely unknown. Identification of sites of frequent and recurring allelic deletion or gain is a first step toward identifying some of the important genes involved in the malignant process. Previous studies in retinoblastoma (Friend, et al. Nature, 323:643-6 (1986)) and other cancers (Cawthon, et al., Cell, 62:193-201 (1990); Baker, et al., Science, 244:217-21 (1989); Shuin, et al., Cancer Res, 54:2832-5 (1994)) have amply demonstrated that...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/16C12Q1/68C40B30/04G01N33/53C12N5/02A61K31/7088A61P35/04C12N15/09A61K38/00A61K45/00A61K48/00A61P35/00
CPCC12Q1/6886C12Q2600/156A61P35/00A61P35/04
Inventor SHAYESTEH, LALEHGRAY, JOE W.MILLS, GORDON B.LU, YILING
Owner RGT UNIV OF CALIFORNIA