Method for preparing direct seeding compression nutrition pot raw material

Abstract

The invention relates to a method for preparing a direct seeding compression nutrition pot raw material. The method comprises the following steps of: inoculating 8 to 12 percent of mycete compound microbial inoculum, 4 to 6 percent of yeast compound microbial inoculum, 6 to 10 percent of azotobacter compound microbial inoculum and 4 to 6 percent of bacillus subtilis compound microbial inoculum into a fermentation culture medium sequentially, and stirring the culture medium repeatedly; and after the fermentation is finished, drying at the temperature of between 40 and 45 DEG C under the vacuum of 0.1 MPa until the moisture is less than or equal to 8 percent, and packaging to obtain products. The direct seeding compression nutrition pot raw material prepared by the method contains bactericides and various nutritional components which are required by the seedling emergence and growth of sprouts, and can ensure the whole seedling and strong seedling of direct root system plants.

Description

technical field

[0001] The invention relates to a method for preparing live compressed nutrition bowl raw materials, in particular to a preparation method for live compressed nutrition bowl raw materials whose active ingredients are composed of various microbial agents. Background technique

[0002] For tap root plants (such as cotton, rapeseed, etc.), in order to solve the difficulty of sowing large planting areas, save seeds, and increase production, it is necessary to transplant seedlings during the planting process (please briefly describe the reasons for transplanting seedlings). It is easy to lose seedlings during the planting process, and the labor intensity is high, so new seedling methods are constantly explored in the seedling raising process. At the end of the 1980s, the technology of "nutritional pot seedling cultivation and transplanting" was widely used in the cotton planting process. For example, Xu Xunyuan ("Cotton Nutrient Bowl Seedling Cultivation Early Qu...

Examples

Embodiment 1

[0026] The preparation of embodiment 1 mold composite bacterial agent

[0027] 1) The preparation process of four single-strain preparations of Aspergillus niger, Aspergillus oryzae, Trichoderma viride and Geotrichum candidum

[0028] Aspergillus niger, Aspergillus oryzae, Trichoderma viride and Geotrichum candidum were inoculated into the Erlenmeyer flask with PDA liquid medium, cultivated at 30°C and 180rpm, so that the number of spores reached 2.5-6.0×10 8 Individual / mL promptly gets seed liquid, by 5-10% inoculum amount, seed liquid is inoculated to the substratum composition ratio and is: stalk 90%, bran 6%, (NH 4 ) 2 SO 4 2%, urea 1%, KH 2 PO 4 0.6%, MgSO 4 0.2%, CaCl 2 0.2%, in a solid-state fermentation medium with a moisture content of 60-70% of the total solid content, ferment at 30°C and turn over every 2-3 hours, and the fermentation product is vacuum-dried to moisture at 40°C ≤10% is a single-strain preparation. The spore content in the bacterial agent is...

Embodiment 2

[0031] The preparation of embodiment 2 yeast compound bacterial agents

[0032] 1) The preparation process of three single-strain preparations of Saccharomyces cerevisiae, Candida tropicalis and Candida utilis

[0033] In complete medium (glucose 2%, yeast extract 1%, peptone 2%, pH natural) solid slant medium, through twice activated Saccharomyces cerevisiae, Candida tropicalis, Candida utilis strains inoculated into the In the Erlenmeyer flask of liquid culture medium with complete medium, cultivate at 28°C and rotate at 180rpm, so that the number of cells reaches 7-9×10 8 Each / mL is the seed solution, inoculate the seed solution into the sterilized wort with a concentration of 8-15Bx according to the inoculum amount of 4-6%, at 28°C, the rotating speed is 180rpm, and the ventilation rate is 0.3-1v / v. Cultivate under the condition of min until the number of cells is 2.5-4.5×10 9 Individual / mL, that is, a single-strain preparation of three yeasts.

[0034] 2) Preparation o...

Embodiment 3

[0036] The preparation of embodiment 3 nitrogen-fixing bacteria composite bacteria agent

[0037] 1) The preparation process of two single-strain preparations of Alcaligenes faecalis and Azotobacter sphaeroides

[0038] In Asbby nitrogen-free medium (mannitol 10g, calcium carbonate 5g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.2g, calcium sulfate 0.1g, distilled water 1000mL, pH6.8-7.0) solid slant medium Inoculate Alcaligenes faecalis and Azotobacter sphaeroides that have been activated twice in the medium into the liquid medium Erlenmeyer flask equipped with Asbby nitrogen-free medium, and cultivate them at 32°C and 180rpm, so that the number of cells reaches 5-7× 10 8 seeds / mL to obtain the seed liquid, inoculate the seed liquid into Asbby nitrogen-free medium according to the inoculum amount of 6-8%, and cultivate until the cells are grown at 32°C, with a rotation speed of 180rpm and an air flow of 0.5-1v / v·min. Number 3-5.5×10 9 Eac...

technical field

[0001] The invention relates to a method for preparing live compressed nutrition bowl raw materials, in particular to a preparation method for live compressed nutrition bowl raw materials whose active ingredients are composed of various microbial agents. Background technique

[0002] For tap root plants (such as cotton, rapeseed, etc.), in order to solve the difficulty of sowing large planting areas, save seeds, and increase production, it is necessary to transplant seedlings during the planting process (please briefly describe the reasons for transplanting seedlings). It is easy to lose seedlings during the planting process, and the labor intensity is high, so new seedling methods are constantly explored in the seedling raising process. At the end of the 1980s, the technology of "nutritional pot seedling cultivation and transplanting" was widely used in the cotton planting process. For example, Xu Xunyuan ("Cotton Nutrient Bowl Seedling Cultivation Early Qu...

Examples

Embodiment 1

[0026] The preparation of embodiment 1 mold composite bacterial agent

[0027] 1) The preparation process of four single-strain preparations of Aspergillus niger, Aspergillus oryzae, Trichoderma viride and Geotrichum candidum

[0028] Aspergillus niger, Aspergillus oryzae, Trichoderma viride and Geotrichum candidum were inoculated into the Erlenmeyer flask with PDA liquid medium, cultivated at 30°C and 180rpm, so that the number of spores reached 2.5-6.0×10 8 Individual / mL promptly gets seed liquid, by 5-10% inoculum amount, seed liquid is inoculated to the substratum composition ratio and is: stalk 90%, bran 6%, (NH 4 ) 2 SO 4 2%, urea 1%, KH 2 PO 4 0.6%, MgSO 4 0.2%, CaCl 2 0.2%, in a solid-state fermentation medium with a moisture content of 60-70% of the total solid content, ferment at 30°C and turn over every 2-3 hours, and the fermentation product is vacuum-dried to moisture at 40°C ≤10% is a single-strain preparation. The spore content in the bacterial agent is...

Embodiment 2

[0031] The preparation of embodiment 2 yeast compound bacterial agents

[0032] 1) The preparation process of three single-strain preparations of Saccharomyces cerevisiae, Candida tropicalis and Candida utilis

[0033] In complete medium (glucose 2%, yeast extract 1%, peptone 2%, pH natural) solid slant medium, through twice activated Saccharomyces cerevisiae, Candida tropicalis, Candida utilis strains inoculated into the In the Erlenmeyer flask of liquid culture medium with complete medium, cultivate at 28°C and rotate at 180rpm, so that the number of cells reaches 7-9×10 8 Each / mL is the seed solution, inoculate the seed solution into the sterilized wort with a concentration of 8-15Bx according to the inoculum amount of 4-6%, at 28°C, the rotating speed is 180rpm, and the ventilation rate is 0.3-1v / v. Cultivate under the condition of min until the number of cells is 2.5-4.5×10 9 Individual / mL, that is, a single-strain preparation of three yeasts.

[0034] 2) Preparation o...

Embodiment 3

[0036] The preparation of embodiment 3 nitrogen-fixing bacteria composite bacteria agent

[0037] 1) The preparation process of two single-strain preparations of Alcaligenes faecalis and Azotobacter sphaeroides

[0038] In Asbby nitrogen-free medium (mannitol 10g, calcium carbonate 5g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.2g, calcium sulfate 0.1g, distilled water 1000mL, pH6.8-7.0) solid slant medium Inoculate Alcaligenes faecalis and Azotobacter sphaeroides that have been activated twice in the medium into the liquid medium Erlenmeyer flask equipped with Asbby nitrogen-free medium, and cultivate them at 32°C and 180rpm, so that the number of cells reaches 5-7× 10 8 seeds / mL to obtain the seed liquid, inoculate the seed liquid into Asbby nitrogen-free medium according to the inoculum amount of 6-8%, and cultivate until the cells are grown at 32°C, with a rotation speed of 180rpm and an air flow of 0.5-1v / v·min. Number 3-5.5×10 9 Eac...

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