Potential sensor for phosphate anions and application of potential sensor

Abstract

The invention belongs to the technical field of analytical tests, and particularly relates to a potential sensor for phosphate anions and application of the potential sensor.According to the potential sensor, the complex (Cu2+-BPMP and Zn2+-BPMP) of Cu2+ or Zn2+ and 2,6-bis{[bis(2-pyridyl methyl)amino]methyl}-4-methylphenol is used as a molecular recognition body, catechol, o-mercaptophenol and derivatives of catechol and o-mercaptophenol are used as indication molecules, and a polymer film electrode doped with anion exchanger is used as an indication electrode.The sensor is high in sensitivity, good in selectivity and free of turbidity and chromaticity interference and has quite good actual application prospects.

Description

Technical field

[0001] The invention belongs to the technical field of analysis and testing, and specifically relates to a phosphate ion potential sensor and its application. Background technique

[0002] Phosphate ions have important physiological and environmental significance. Abnormal phosphate ion concentration in body fluids can be used as a sign of disease and metabolic dysfunction, and excessive phosphate in environmental waters can cause eutrophication of the water. The traditional method for detecting phosphate is ammonium molybdate spectrophotometry, which has high accuracy but is easily interfered by turbidity and color. In addition, most of the currently developed phosphate detection methods rely on large-scale laboratory instruments. These problems all lead to the fact that the existing methods cannot be widely used in the on-site rapid detection of environmental water bodies. It is still a difficult problem to develop a simple, selective, and not interfered by t...

Examples

Embodiment 1

[0024] Take Cu 2+ -BPMP is the molecular recognition body to detect phosphate ion as an example. The steps are as follows:

[0025] (1) Preparation of polymer membrane electrode

[0026] Weigh 196 mg of polyvinyl chloride, 196 mg of o-nitrophenyl octyl ether, and 4.0 mg of tridodecyl monomethyl ammonium chloride in 3.0 mL of tetrahydrofuran, stir well, and pour into the glass plate with an inner diameter of 3.5 cm In the glass ring. After the tetrahydrofuran evaporates, use a puncher to cut the membrane into small discs with an inner diameter of 3mm and paste them onto the pipette tip with a PVC tube at the bottom. Use the HEPES solution (containing 10 mM NaCl) with pH=7.0 as the inner filling solution and the activation solution to activate the electrode, and it will be used.

[0027] (2) Assembly of molecular recognition body and indicator molecule

[0028] Use 20mMHEPES buffer solution to prepare 200μM copper perchlorate solution, 100μM H-BPMP solution, mix the two in equal volu...

Embodiment 2

[0036] Take Zn 2+ -BPMP is the molecular recognition body to detect phosphate ion as an example. The steps are as follows:

[0037] (1) Preparation of polymer membrane electrode

[0038] Weigh 196 mg of polyvinyl chloride, 196 mg of o-nitrophenyl octyl ether, and 4.0 mg of tridodecyl monomethyl ammonium chloride in 3.0 mL of tetrahydrofuran, stir well, and pour into the glass plate with an inner diameter of 3.5 cm In the glass ring. After the tetrahydrofuran evaporates, use a puncher to cut the membrane into small discs with an inner diameter of 3mm and paste them onto the pipette tip with a PVC tube at the bottom. Use the HEPES solution (containing 10 mM NaCl) with pH=7.0 as the inner filling solution and the activation solution to activate the electrode, and it will be used.

[0039] (2) Assembly of molecular recognition body and indicator molecule

[0040] Use 20mMHEPES buffer solution to prepare 200μM zinc perchlorate solution, 100μM H-BPMP solution, mix the two in equal volume...

Technical field

[0001] The invention belongs to the technical field of analysis and testing, and specifically relates to a phosphate ion potential sensor and its application. Background technique

[0002] Phosphate ions have important physiological and environmental significance. Abnormal phosphate ion concentration in body fluids can be used as a sign of disease and metabolic dysfunction, and excessive phosphate in environmental waters can cause eutrophication of the water. The traditional method for detecting phosphate is ammonium molybdate spectrophotometry, which has high accuracy but is easily interfered by turbidity and color. In addition, most of the currently developed phosphate detection methods rely on large-scale laboratory instruments. These problems all lead to the fact that the existing methods cannot be widely used in the on-site rapid detection of environmental water bodies. It is still a difficult problem to develop a simple, selective, and not interfered by t...

Examples

Embodiment 1

[0024] Take Cu 2+ -BPMP is the molecular recognition body to detect phosphate ion as an example. The steps are as follows:

[0025] (1) Preparation of polymer membrane electrode

[0026] Weigh 196 mg of polyvinyl chloride, 196 mg of o-nitrophenyl octyl ether, and 4.0 mg of tridodecyl monomethyl ammonium chloride in 3.0 mL of tetrahydrofuran, stir well, and pour into the glass plate with an inner diameter of 3.5 cm In the glass ring. After the tetrahydrofuran evaporates, use a puncher to cut the membrane into small discs with an inner diameter of 3mm and paste them onto the pipette tip with a PVC tube at the bottom. Use the HEPES solution (containing 10 mM NaCl) with pH=7.0 as the inner filling solution and the activation solution to activate the electrode, and it will be used.

[0027] (2) Assembly of molecular recognition body and indicator molecule

[0028] Use 20mMHEPES buffer solution to prepare 200μM copper perchlorate solution, 100μM H-BPMP solution, mix the two in equal volu...

Embodiment 2

[0036] Take Zn 2+ -BPMP is the molecular recognition body to detect phosphate ion as an example. The steps are as follows:

[0037] (1) Preparation of polymer membrane electrode

[0038] Weigh 196 mg of polyvinyl chloride, 196 mg of o-nitrophenyl octyl ether, and 4.0 mg of tridodecyl monomethyl ammonium chloride in 3.0 mL of tetrahydrofuran, stir well, and pour into the glass plate with an inner diameter of 3.5 cm In the glass ring. After the tetrahydrofuran evaporates, use a puncher to cut the membrane into small discs with an inner diameter of 3mm and paste them onto the pipette tip with a PVC tube at the bottom. Use the HEPES solution (containing 10 mM NaCl) with pH=7.0 as the inner filling solution and the activation solution to activate the electrode, and it will be used.

[0039] (2) Assembly of molecular recognition body and indicator molecule

[0040] Use 20mMHEPES buffer solution to prepare 200μM zinc perchlorate solution, 100μM H-BPMP solution, mix the two in equal volume...

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