Preparation technology and application of a new imprinted material of composite metal polyphenols co-complex

Abstract

The invention discloses a microwave irradiation preparation technology and use of new composite metal polyphenol co-complex imprinting materials. According to the method, a mixture of chlorogenic acid, caffeic acid, vanillic acid and protocatechuic acid is taken as template molecules, and the new composite metal polyphenol co-complex imprinting materials are prepared by means of microwave assistance; the reaction time is short; the obtained new imprinting materials are high in imprinting efficiency and stable in imprinting sites, and have stronger molecular acting force with polyphenol compounds; the molecular recognition efficiency and application ability of extracting active parts of plants of the new imprinting materials are far higher than those of the conventional imprinting polymer,so that scientific and technological progress is effectively promoted, and optional efficient absorbents are provided for separating the active parts of the polyphenol compounds; after being used fora single time, the new composite metal polyphenol co-complex imprinting materials are high in target product recovery rate, have higher selectivity for target molecules, have multiple advantages whichare not available in other methods, and are high in practical value.

Description

technical field

[0001] The invention relates to a microwave irradiation preparation technology and application of a composite metal polyphenol co-complex imprinting new material. Background technique

[0002] When traditional Chinese medicine treats diseases, it is often based on the comprehensive treatment and conditioning of the human body by traditional Chinese medicine compound, which has synergistic regulation and therapeutic efficacy that Western medicine does not have. The analysis of drug components shows that the powerful therapeutic effects of traditional Chinese medicine compound are mainly derived from the active parts in traditional Chinese medicine, that is, the synergistic mechanism of the same class of compounds in traditional Chinese medicine in the treatment of diseases. The therapeutic effect of multiple components is often much higher than that of single component bioactive compounds. In order to highlight the curative effect of traditional Chinese medic...

Examples

Embodiment 1

[0016] (1) Weigh Tb(NO 3 ) 3 ·6H 2 O and MnSO 4 , dissolved in 100mL deionized water, slowly drop NaOH solution while stirring, until the pH is 8.5-11, stir, age at room temperature for 5-8h, filter, wash the precipitate with deionized water until neutral, at 60°C Dry for 24 hours and set aside;

[0017] (2) Add 0.5-1.0 mmol of chlorogenic acid-caffeic acid-vanillic acid-protocatechuic acid mixture in a molar ratio of 1:2:2:1, 0.4 mL dimethyl sulfoxide (DMSO), 6.0mL of acetonitrile-tetrahydrofuran mixed solvent (volume ratio 1:2), ultrasonically mixed, slightly heated, and 1.2g of the precipitate obtained in step (1) and 2.0mL of additives (0.34mol / L HF- NaF buffer solution), stirred and allowed to stand for 2-3h to obtain a composite metal-polyphenol complex solution;

[0018] (3) Add 1.6 mmol of acrylamide-dopamine mixed functional monomer (molar ratio 1:8) to the solution obtained in step (2), sonicate for 10 minutes, then add 9 mg of azobisisobutyronitrile (AIBN), and...

Embodiment 2

[0022] (1) Weigh Tb(NO 3 ) 3 ·6H 2 O and MnSO 4 , dissolved in 100mL deionized water, slowly drop NaOH solution while stirring, until the pH is 8.5-11, stir, age at room temperature for 5-8h, filter, wash the precipitate with deionized water until neutral, at 60°C Dry for 24 hours and set aside;

[0023] (2) Add 0.5-1.0 mmol of chlorogenic acid-caffeic acid-vanillic acid-protocatechuic acid mixture in a molar ratio of 1:2:2:1, 0.4 mL dimethyl sulfoxide (DMSO), 6.0mL of acetonitrile-tetrahydrofuran mixed solvent (volume ratio 1:2), ultrasonically mixed, slightly heated, 1.5g of the precipitate obtained in step (1) and 1.7mL of auxiliary ingredients (0.34mol / L HF- NaF buffer solution), stirred and allowed to stand for 2-3h to obtain a composite metal-polyphenol complex solution;

[0024] (3) Add 1.6 mmol of acrylamide-dopamine mixed functional monomer (molar ratio 1:9) to the solution obtained in step (2), sonicate for 10 minutes, then add 9 mg of azobisisobutyronitrile (AI...

technical field

[0001] The invention relates to a microwave irradiation preparation technology and application of a composite metal polyphenol co-complex imprinting new material. Background technique

[0002] When traditional Chinese medicine treats diseases, it is often based on the comprehensive treatment and conditioning of the human body by traditional Chinese medicine compound, which has synergistic regulation and therapeutic efficacy that Western medicine does not have. The analysis of drug components shows that the powerful therapeutic effects of traditional Chinese medicine compound are mainly derived from the active parts in traditional Chinese medicine, that is, the synergistic mechanism of the same class of compounds in traditional Chinese medicine in the treatment of diseases. The therapeutic effect of multiple components is often much higher than that of single component bioactive compounds. In order to highlight the curative effect of traditional Chinese medic...

Examples

Embodiment 1

[0016] (1) Weigh Tb(NO 3 ) 3 ·6H 2 O and MnSO 4 , dissolved in 100mL deionized water, slowly drop NaOH solution while stirring, until the pH is 8.5-11, stir, age at room temperature for 5-8h, filter, wash the precipitate with deionized water until neutral, at 60°C Dry for 24 hours and set aside;

[0017] (2) Add 0.5-1.0 mmol of chlorogenic acid-caffeic acid-vanillic acid-protocatechuic acid mixture in a molar ratio of 1:2:2:1, 0.4 mL dimethyl sulfoxide (DMSO), 6.0mL of acetonitrile-tetrahydrofuran mixed solvent (volume ratio 1:2), ultrasonically mixed, slightly heated, and 1.2g of the precipitate obtained in step (1) and 2.0mL of additives (0.34mol / L HF- NaF buffer solution), stirred and allowed to stand for 2-3h to obtain a composite metal-polyphenol complex solution;

[0018] (3) Add 1.6 mmol of acrylamide-dopamine mixed functional monomer (molar ratio 1:8) to the solution obtained in step (2), sonicate for 10 minutes, then add 9 mg of azobisisobutyronitrile (AIBN), and...

Embodiment 2

[0022] (1) Weigh Tb(NO 3 ) 3 ·6H 2 O and MnSO 4 , dissolved in 100mL deionized water, slowly drop NaOH solution while stirring, until the pH is 8.5-11, stir, age at room temperature for 5-8h, filter, wash the precipitate with deionized water until neutral, at 60°C Dry for 24 hours and set aside;

[0023] (2) Add 0.5-1.0 mmol of chlorogenic acid-caffeic acid-vanillic acid-protocatechuic acid mixture in a molar ratio of 1:2:2:1, 0.4 mL dimethyl sulfoxide (DMSO), 6.0mL of acetonitrile-tetrahydrofuran mixed solvent (volume ratio 1:2), ultrasonically mixed, slightly heated, 1.5g of the precipitate obtained in step (1) and 1.7mL of auxiliary ingredients (0.34mol / L HF- NaF buffer solution), stirred and allowed to stand for 2-3h to obtain a composite metal-polyphenol complex solution;

[0024] (3) Add 1.6 mmol of acrylamide-dopamine mixed functional monomer (molar ratio 1:9) to the solution obtained in step (2), sonicate for 10 minutes, then add 9 mg of azobisisobutyronitrile (AI...