Preparation method and application of blended hydrogel biological scaffold material with biological activity

Abstract

The invention belongs to the field of biological materials for tissue engineering repair and tissue regeneration promotion, and particularly relates to a preparation method and application of a blended hydrogel biological scaffold material with biological activity. The blended hydrogel biological scaffold material is prepared by sequentially mixing an acellular bone meal matrix and methacrylate-modified gelatin according to a certain proportion. The preparation method of the scaffold material provided by the invention is safe, non-toxic and easy to operate, the acellular bone meal with low immunogenicity is obtained by performing acellular treatment on bones of alpha-1,3-galactoside gene knockout pigs, and the acellular bone meal has the characteristic of low immunogenicity after being implanted into a living body. The tissue engineering scaffold material is obtained by blending the acellular bone meal with biological activity and low immunogenicity with the GelMA hydrogel, the hydrogel material mixed with the acellular bone meal reserves the extracellular matrix of a natural material, the hydrogel can also provide a good supporting effect for the scaffold material, the biocompatibility is good, and thereby the scaffold material can be used as a bone tissue engineering material to be applied to hard bone defects in vivo, skull repair and the like. Compared with a traditional treatment method, the scaffold material has wide application prospects.

Description

technical field

[0001] The invention belongs to the biological field of tissue repair and regeneration, and in particular relates to the preparation and application of a bioactive blended hydrogel bio-scaffold material. Background technique

[0002] Tissue engineering is a comprehensive emerging discipline based on the development and application of chemistry, physics, engineering, life and clinical medicine, dedicated to solving medical problems such as tissue defect and organ failure. It encompasses the most fundamental understanding of the structure and function of normal and diseased tissues, and the use of biological substitutes to recreate, maintain or promote functional expression of tissues. In the human body, various biophysical and biochemical stimuli control its growth and development process, and maintain the physiological structure and function of tissues and organs. Therefore, we must understand and be good at using these promoting factors to simulate tissue en...

Examples

Embodiment 1

[0034] Such as Figure 1 to Figure 5 As shown, the invention discloses a preparation method of a bioactive blended hydrogel bioscaffold material,

[0035] (1) Weigh 20% w / v GelMA solid, add it to a 5ml centrifuge tube containing 2ml deionized water, dissolve in a 37°C water bath, shake and mix;

[0036] (2) Weigh 5% decellularized bone powder, add it to the dissolved solution in step (1), dissolve in a 37°C water bath, shake and mix;

[0037] (3) Weigh 0.1% w / v photoinitiated crosslinking agent Ig2959, add it to the mixed solution in step (2), shake and mix well; the photoinitiated crosslinking agent is 2-hydroxy-2-methyl-1- [4-(2-Hydroxyethoxy)phenyl]-1-propanone.

[0038] (4) Clean the polytetrafluoroethylene mold with a diameter of 8 mm and a depth of 1 mm, and dry it for use.

[0039] (5) Drop 60 μl of the mixed solution prepared in step (3) into the polytetrafluoroethylene mold prepared in step (4);

[0040] (6) Place the material prepared in step (5) under a 365nm ul...

Embodiment 2

[0043] (1) Weigh 20% w / v GelMA solid, add it to a 5ml centrifuge tube containing 2ml deionized water, dissolve in a 37°C water bath, shake and mix;

[0044] (2) Weigh 20% of the decellularized bone powder, add it to the dissolved solution in step (1), dissolve in a 37°C water bath, shake and mix;

[0045](3) Weigh 0.1% w / v photoinitiated cross-linking agent Ig2959, add it to the mixed solution in step (2), shake and mix;

[0046] (4) Clean the polytetrafluoroethylene mold with a diameter of 8 mm and a depth of 1 mm, and dry it for use.

[0047] (5) Drop 60 μl of the mixed solution prepared in step (3) into the polytetrafluoroethylene mold prepared in step (4);

[0048] (6) Place the material prepared in step (5) under a 365nm ultraviolet lamp and irradiate it for 2 minutes, and the photocrosslinking is completed;

[0049] (7) The material obtained in step (6) is soaked in the buffer solution and stored.

Embodiment 3

[0051] (1) Weigh 20% w / v GelMA solid, add it to a 5ml centrifuge tube containing 2ml deionized water, dissolve in a 37°C water bath, shake and mix;

[0052] (2) Weigh 35% decellularized bone powder, add it to the dissolved solution in step (1), dissolve in a 37°C water bath, shake and mix;

[0053] (3) Weigh 0.1% w / v photoinitiated cross-linking agent Ig2959, add it to the mixed solution in step (2), shake and mix;

[0054] (4) Clean the polytetrafluoroethylene mold with a diameter of 8 mm and a depth of 1 mm, and dry it for use.

[0055] (5) Drop 60 μl of the mixed solution prepared in step (3) into the polytetrafluoroethylene mold prepared in step (4);

[0056] (6) Place the material prepared in step (5) under a 365nm ultraviolet lamp and irradiate it for 2 minutes, and the photocrosslinking is completed;

[0057] (7) The material obtained in step (6) is soaked in the buffer solution and stored.

technical field

[0001] The invention belongs to the biological field of tissue repair and regeneration, and in particular relates to the preparation and application of a bioactive blended hydrogel bio-scaffold material. Background technique

[0002] Tissue engineering is a comprehensive emerging discipline based on the development and application of chemistry, physics, engineering, life and clinical medicine, dedicated to solving medical problems such as tissue defect and organ failure. It encompasses the most fundamental understanding of the structure and function of normal and diseased tissues, and the use of biological substitutes to recreate, maintain or promote functional expression of tissues. In the human body, various biophysical and biochemical stimuli control its growth and development process, and maintain the physiological structure and function of tissues and organs. Therefore, we must understand and be good at using these promoting factors to simulate tissue en...

Examples

Embodiment 1

[0034] Such as Figure 1 to Figure 5 As shown, the invention discloses a preparation method of a bioactive blended hydrogel bioscaffold material,

[0035] (1) Weigh 20% w / v GelMA solid, add it to a 5ml centrifuge tube containing 2ml deionized water, dissolve in a 37°C water bath, shake and mix;

[0036] (2) Weigh 5% decellularized bone powder, add it to the dissolved solution in step (1), dissolve in a 37°C water bath, shake and mix;

[0037] (3) Weigh 0.1% w / v photoinitiated crosslinking agent Ig2959, add it to the mixed solution in step (2), shake and mix well; the photoinitiated crosslinking agent is 2-hydroxy-2-methyl-1- [4-(2-Hydroxyethoxy)phenyl]-1-propanone.

[0038] (4) Clean the polytetrafluoroethylene mold with a diameter of 8 mm and a depth of 1 mm, and dry it for use.

[0039] (5) Drop 60 μl of the mixed solution prepared in step (3) into the polytetrafluoroethylene mold prepared in step (4);

[0040] (6) Place the material prepared in step (5) under a 365nm ul...

Embodiment 2

[0043] (1) Weigh 20% w / v GelMA solid, add it to a 5ml centrifuge tube containing 2ml deionized water, dissolve in a 37°C water bath, shake and mix;

[0044] (2) Weigh 20% of the decellularized bone powder, add it to the dissolved solution in step (1), dissolve in a 37°C water bath, shake and mix;

[0045](3) Weigh 0.1% w / v photoinitiated cross-linking agent Ig2959, add it to the mixed solution in step (2), shake and mix;

[0046] (4) Clean the polytetrafluoroethylene mold with a diameter of 8 mm and a depth of 1 mm, and dry it for use.

[0047] (5) Drop 60 μl of the mixed solution prepared in step (3) into the polytetrafluoroethylene mold prepared in step (4);

[0048] (6) Place the material prepared in step (5) under a 365nm ultraviolet lamp and irradiate it for 2 minutes, and the photocrosslinking is completed;

[0049] (7) The material obtained in step (6) is soaked in the buffer solution and stored.

Embodiment 3

[0051] (1) Weigh 20% w / v GelMA solid, add it to a 5ml centrifuge tube containing 2ml deionized water, dissolve in a 37°C water bath, shake and mix;

[0052] (2) Weigh 35% decellularized bone powder, add it to the dissolved solution in step (1), dissolve in a 37°C water bath, shake and mix;

[0053] (3) Weigh 0.1% w / v photoinitiated cross-linking agent Ig2959, add it to the mixed solution in step (2), shake and mix;

[0054] (4) Clean the polytetrafluoroethylene mold with a diameter of 8 mm and a depth of 1 mm, and dry it for use.

[0055] (5) Drop 60 μl of the mixed solution prepared in step (3) into the polytetrafluoroethylene mold prepared in step (4);

[0056] (6) Place the material prepared in step (5) under a 365nm ultraviolet lamp and irradiate it for 2 minutes, and the photocrosslinking is completed;

[0057] (7) The material obtained in step (6) is soaked in the buffer solution and stored.

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