The invention discloses a preparation method for extracting water-soluble polyose from black pine needles according to a decompression inner-boiling method. The preparation method comprises the following steps: putting the black pine needles in a constant-temperature
drying cabinet for 24 hours'
drying at 40 DEG C, milling the dried black pine needles by a miller, and sieving with a 80-mesh
sieve; weighing 1 g of the obtained black pine needle
powder, putting the weighed black pine needle
powder in a glass device, and adding 2 ml of
ethanol for 30 minutes' uniform
wetting, so as to ensure that
ethanol permeates the black pine needle
powder; carrying out vacuum suction to reduce the gauge pressure to -0.080 MPa, carrying out decompression suction till slightly boiled bubbles are generated on the mixture surface, and carrying out
filtration; extracting a solution by utilizing hot water of which the volume is 20 times of that of the solution and the temperature is 85 DEG C for 6 minutes; selecting a small amount of the extracted solution for
dilution, and measuring the polyose content according to a
phenol-
sulfuric acid method; precipitating the extracted solution by utilizing a 4%
ethanol solution, carrying out still standing for a night, carrying out
centrifugation, and then taking out the precipitate for
drying in the drying box; preparing the dried solution into a polyose product. Through adoption of the technical scheme, the leaching amount of polyose is improved; the decompression inner-boiling method is low in production cost and simple in process; the preparation method has the advantages that the effective components of polyose can be quickly extracted and separated in an environment-friendly manner.